Glycosylation state and target protein identity in complex protein samples examined in new poster from LI-COR Biosciences

November 11, 2005: Researchers from LI-COR Biosciences presented new information on a multiplexed method to glean information about both glycosylation state and target protein identity in complex protein samples.   The poster, Lectin-Based Detection of Carbohydrates with Near-Infrared Flurophores uses infrared fluorescent detection to bypass the need for colorimetric or chemiluminescent reagents and produces more quantitative results than enzymatic systems. Data was presented at the Society of Glycobiology meeting November 11 in Boston, Massachusetts.   A link to download a copy of this poster in PDF form is available at the end of this article.

Highlights of the poster include:

• Detection of glycoproteins with infrared dyes and the Odyssey® System were accurate and highly sensitive, with detection limits in the 4-8 ng range.
• A multiplexed fluorescent method was developed. This method yields information about glycosylation state (using lectin-based detection) and also confirms the identity of the glycoprotein (using a specific antibody). Carbohydrate composition can be dissected using multiple labeled lectins.
• Biotinylated lectins and IRDye® 800CW streptavidin are a ready-to-use solution for carbohydrate detection, and directly-labeled lectins can also be generated.

For additional information on this poster, contact: Joseph Hwang, (jhwang@licor.com) or LI-COR Product Manager for Odyssey Neeraj Asundi (nasundi@licor.com)

Click here to download a PDF of the poster.

The Odyssey Infrared Imaging System is uniquely equipped with two infrared channels for direct fluorescence detection on membranes with sensitivity that is equal to or better than chemiluminescence. The superior signal-to-noise ratio produced using near infrared dyes produces results not possible with traditional fluorescence-based imaging systems. LI-COR infrared systems are noted for providing superior imaging for a growing list of applications including Western Blots, In-Cell Westerns, EMSA’s, Gel Documentation and In Vivo imaging.