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EMSA Oligos & Buffer Kit

More Applications

2-D Gel Documentation In-Cell Westerns Infrared EMSA Analysis in vivo Imaging Northern Blots Protein Phosphorylation Two-Color In-Gel Westerns Protein Gel Documentation Two-Color Western Blots

Infrared EMSA Analysis

Infrared AP-1 EMSA was used to visualize the changes in AP-1 binding following three different treatments of Hela cells. Hela, Hela 2 hour serum response, and Hela 4 hour serum response nuclear extracts were serially diluted and assayed under the same conditions using IRDye®700 end-labeled oligonucleotide duplex containing AP-1 binding sequence. Following electrophoresis the gel was imaged while remaining in the glass plates.

Existing mobility shift assay protocols can be easily transformed into infrared assays by replacing the existing oligonucleotides with IRDye® end-labeled oligonucleotides. The binding conditions and electrophoresis conditions should remain the same as with any other EMSA detection method but may require some optimization.