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In-Cell Western Immunofluorescent Assays

Advantages

  • Simultaneous, two-target detection enables precise quantification and accurate measurement of abundance or phosphorylation of one target by normalization against another target or DNA stain.
  • Direct detection of proteins in their cellular context eliminates variabilities and artifacts caused by cell lysis. In-cell detection can provide more relevant results than enzyme assays with purified proteins.
  • Near-infrared probes yield high sensitivity for measuring small changes in protein amount or modification.
  • Analyzes multiple targets in 96- or 384-well plates.
  • Fast, microplate-based assay – lysate preparation, gel loading, electrophoresis, and membrane transfer are eliminated.
  • Ideal for screening cell treatments or drug candidates for effects on target proteins.
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