Biotechnology
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| The Odyssey system uses two infrared fluorescence channels for detection, enabling simultaneous two-color target analysis – an advantage that’s not available with chemiluminescent or radioactive methods. Two-color Western analysis makes normalization easy and eliminates error introduced by stripping and reprobing or by comparison of separate blots. Superior image clarity and detail makes it easier to detect subtle mobility shifts caused by protein modifications such as phosphorylation. | |
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| ERK1/2 and phospho-ERK were detected simultaneously in lysates of unstimulated and EGF-stimulated A431 cells. Two fold serial dilutions of lysate are shown. The single-color images can be overlaid to show both total ERK and phospho-ERK (yellow color indicates overlap of red and green signals). The mobility shift caused by phosphorylation can be seen in the EGF-stimulated lysate. | |
