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Biotechnology
The Odyssey system uses two infrared fluorescence channels
for detection, enabling simultaneous two-color target analysis – an
advantage that’s not available with chemiluminescent
or radioactive methods. Two-color Western analysis makes
normalization easy and eliminates error introduced by stripping
and reprobing or by comparison of separate blots. Superior
image clarity and detail makes it easier to detect subtle
mobility shifts caused by protein modifications such as
phosphorylation. |
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| ERK1/2 and phospho-ERK were detected simultaneously in
lysates of unstimulated and EGF-stimulated A431 cells.
Two fold serial dilutions of lysate are shown. The single-color
images can be overlaid to show both total ERK and phospho-ERK
(yellow color indicates overlap of red and green signals).
The mobility shift caused by phosphorylation can be seen
in the EGF-stimulated lysate. |
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