The Infrared Advantage
prevnext
Through its innovative use of fluorescently labeled antibodies rather than enzyme labels, Odyssey provides a broad, linear dynamic range to accurately detect strong and weak bands on the same Western blot. In contrast, the dynamic enzymatic nature of chemiluminescence only allows you to capture a “snapshot” of the enzymatic reaction and is highly dependent on timing and exposure, limiting linear range and offering only qualitative or semi-quantitative results. The accuracy and linearity of Odyssey detection allow you to be confident about differences you see in protein levels, and your blots can be archived and imaged again months later, if needed.
Quantification
Two-fold serial dilutions of Alexa® 680-labeled antibody (6 ng to 0.19 pg) were spotted on nitrocellulose and imaged in the 700 nm channel.
Quantification

 

prevnext
x
A A A
line
800 | 1024 | max
Biotechnology
4647 Superior St
Lincoln, NE 68504
Toll-Free: 800-645-4267
Email: biohelp@licor.com
Environmental
4421 Superior Street
Lincoln, NE 68504
Toll-Free: 800-447-3576
Email: envsales@licor.com