The Infrared Advantage
prevnext
Two separate lasers and detectors simultaneously detect both fluorescent signals. The optical system employs diode lasers and solid-state detectors due to their long lifetimes and very low maintenance requirements. Infrared laser excitation outperforms systems that use white light and filter wheels by delivering higher intensity excitation light to the fluorophore. A variety of fluorescent dyes and stains are compatible with the 685 and 785 nm excitation wavelengths of Odyssey’s two diode lasers. Spectral overlap is minimized by the 100 nm separation of the two detection channels, and optical filtering assures that each detector measures fluorescence from only one of the infrared dyes.
Odyssey Cutaway Drawing
Two solid-state diode lasers (A) simultaneously provide excitation at 680 and 780 nm. Both laser beams are focused to form an excitation spot on the scanning surface. The microscope objective (B) is focused on the excitation spot and collects light from both fluorescing infrared dyes. Resolution is user-adjustable from 21 to 336 µm. Light from the microscope objective is passed to a dichroic mirror (C) that splits the light into two fluorescent signals by transmitting light above 810 nm and reflecting light below 750 nm. The transmitted and reflected light travels two independent optical paths and is finally focused on separate silicon avalanche photodiodes (D) and detected.
prevnext
x
A A A
line
800 | 1024 | max
Biotechnology
4647 Superior St
Lincoln, NE 68504
Toll-Free: 800-645-4267
Email: biohelp@licor.com
Environmental
4421 Superior Street
Lincoln, NE 68504
Toll-Free: 800-447-3576
Email: envsales@licor.com