Infrared Detection vs. Chemiluminescence

“LI-COR has brought down the costs of protein analysis by introducing an approach that takes advantage of the inherent sensitivity advantage of the near-infrared spectrum, thereby removing the need to develop film while performing immunoblots (a time-consuming, labor-intensive operation).

“Compared with traditional systems, the cost of performing an identical protein analysis experiment using the Odyssey system is 90% less. This system’s fluorescent detection and digital delivery of data improves its usefulness by providing quantitative numerical results, rather than prior methods that provided purely qualitative images.”

--Frost & Sullivan, September 25, 2006

 

Cost Comparison Table

Reagents
Typical Costs For A Single 10x10 Blot
IR Detection
(2 color)
Chemiluminescence
(1 color)
Chemiluminescence
(Strip and Reprobe)
Calculate
Your Savings
Secondary Antibody
Recommended Dilutions:
1:15000 for IR; 1:2500 for Chemi
$0.82 $0.10 $0.20

Chemiluminescent Reagents (12mL) $0.00 $12.97 $25.93

Film (2 Films/blot) $0.00 $5.76 $5.76

Protein Markers
Recommended Amount:
1uL for IR; 10uL for Chemi

$0.22 $4.84 $4.84

         
Cost: $1.04 $23.67 $36.73 $ -

         
      # of Blots/week
weekly savings...
$ -
     
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*Based on typical list prices of chemiluminescent reagents as of October 15, 2006.

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