next

Near-Infrared Detection for the Highest Sensitivity

» The Pearl Imager uses a new, proprietary CCD-based optical system specifically designed for in vivo imaging in the near-infrared (NIR) spectral region where tissue autofluorescence and light scattering are low.
» Patented filtering design dramatically reduces noise.
»

Signal detection is optimized for industry leading IRDye® near-infrared dyes.

signal detection
Molar extinction coefficient characteristics of water, hemoglobin and oxygenated hemoglobin (400-1000 nm). IRDye 800CW has ideal excitation/emission wavelengths for in vivo imaging.
» NIR laser illumination permits much deeper tissue penetration than white light. Smaller targets can be visualized at greater depths, allowing earlier tumor detection.
» Requires less than 30 seconds to capture white light, 700 nm, and 800 nm images. Speed minimizes animal stress and increases productivity.
»

Dual channel detection in a single image acquisition.

Mouse scan
Dual channel detection: In the 800 nm channel, IRDye 800CW 2-DG Optical Probe was used for tumor detection and is represented in pseudocolor. IRDye 680 BoneTag™ was used to visualize skeletal structures in the 700 nm channel (grayscale), aiding in anatomical localization of the tumor.
» Portable. Small footprint simplifies mobility and accomodates placement into any lab.
» Six logs (22 bits) of dynamic range available for each image: Up to 4 logs of usable dynamic range when imaging mice in the 800 nm channel, or up to 6 logs of dynamic range when imaging excised tumors or organs.
 
IRDye Signal Chart
To illustrate the wide dynamic range of the detection system, a series of tubes containing increasing concentrations of IRDye 800CW (0-10 μM) was imaged. Signal represents the total fluorescence for a region of interest minus background fluorescence for the region.
Tumor Screen
Tumor screen 3 days after injection of IRDye 800CW EGF Optical Probe (green). The three tumors on the left are from control animals. Red signal is autofluorescence.
next
corner corner

Key Publications

» Seeing Beyond the Visible With IRDye® Infrared Dyes pdf
» A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models pdf
» Hyaluronidase Expression Induces Prostate Tumor Metastasis in an Orthotopic Mouse Model (Abstract)
» Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer (Abstract)
   
x
A A A
line
800 | 1024 | max
Biotechnology
4647 Superior St
Lincoln, NE 68504
Toll-Free: 800-645-4267
Email: biohelp@licor.com
Environmental
4421 Superior Street
Lincoln, NE 68504
Toll-Free: 800-447-3576
Email: envsales@licor.com