Sequencing BAC DNA With Near-Infrared
Fluorescent Non-Nucleotide Terminators

Stephen C. Roemer, Vince R. Boveia, Craig M. Johnson and D. Michael Olive
LI-COR Inc., Biotechnology, 4647 Superior Street, Lincoln, NE 68504

Sequencing Tables

BAC sequencing using IRDye® infrared dye-labeled AcycloTerminators and
IRDye® infrared dye-labeled primers. Three BAC templates are shown for
comparison of read length and accuracy.

Sample Designation	Template Size	Sequencing Method	Enzyme	Read Length	Q Value
101	100 Kb	Dye Terminator	Thermo Sequenase	1195	29
101	100 Kb	Dye Primer	Thermo Sequenase	934	38
102	100 Kb	Dye Terminator	Thermo Sequenase	1119	33
102	100 Kb	Dye Primer	Thermo Sequenase	1147	37
202	70 Kb	Dye Terminator	Thermo Sequenase	809	22
202	70 Kb	Dye Primer	Thermo Sequenase	264	31

Polymerase effects on difficult BAC sequences.

The performance of Thermo Sequenase^® was compared to SequiTherm
Excel™ II using IRDye® infrared dye-labeled primers for sequencing three
unusually difficult BAC templates.

Sample Designation	Template Size	Sequencing Method	Enzyme	Read Length	Q Value
201	105 Kb	Dye Primer	Thermo Sequenase	584	19
201	105 Kb	Dye Primer	Excel II	1137	32
202	70 Kb	Dye Primer	Thermo Sequenase	264	21
202	70 Kb	Dye Primer	Excel II	1107	31
203	80 Kb	Dye Primer	Thermo Sequenase	594	20
203	80 Kb	Dye Primer	Excel II	1137	28