Biotechnology
| Poster #548 | |
| » | Abstract |
| » | General Class Information |
| » | Project Background |
| » | Assessment |
| » | Extensions of Curriculum |
| » | Molecular Biology Students |
| » | Funding Acknowledgements |
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Bacterial Diversity Studies Using the 16S rRna Gene Provide a Powerful Research-Based Curriculum for Molecular Biology Laboratory
S. Boomer, D. Lodge, and B. Dutton (Western Oregon University)
General Class Information
Molecular Biology (Bio475) is open to majors and minors in biology. Pre-requisites include one year of 200-level introductory biology and chemistry. Cell Biology (Bio388), Microbiology (Bio331), and Genetics (Bio341) are strongly recommended. Labs last 4 hours a week; students also attend 2 hours of lecture per week (total class credit = 4). The course is offered every spring and typically serves 4-8 students. The last 2 years have involved this specific project and are described.
Syllabus in Brief
| UNIT ONE
PLASMIDS |
Week 1: Large-Scale Isolation I Week 2: Large-Scale Isolation IIa |
[Details]
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| UNIT TWO
RESTRICTION ENZYMES |
Week 3: Diversity Assessment Week 6: DNA Sequence Analysis IIa |
[Details]
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| UNIT THREE
DNA SEQUENCING |
Week 5: DNA Sequence Analysis I Week 6: DNA Sequence Analysis IIa |
[Details]
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| UNIT FOUR
CLONING/PCR |
Week 7: Editing, Assembly, BLAST Week 8: Phylogenetics, Databasesa,b |
[Details]
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| UNIT FIVE
CLONING/PCR |
Week 9: Genomic DNA, PCR, Cloning Week 10: Screening a Librarya,b,c |
[Details]
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a) Formal Laboratory Notebooks Collected (50 pts.) and Quizzes (30 pts.)
b) Formal Phylogenetics Report (75 pts.)
c) Research-Style Poster Panel Due for Team Presentation (75 pts.)
