Unit Two

Restriction Analysis of 16S Clones

CONCEPTS
Biology
-Origin and function of restriction enzymes
-DNA variation, origins and implications

Chemistry
-Chemistry and topology of DNA
-Principles of gel electrophoresis
-Enzyme optima and buffer selection

METHODS & QUANTITATIVE SKILLS
-Calculations and dilutions
-Precise pipetting and measurement
-Graphing DNA mobilities
-Mapping unknown plasmids
-Safely handling ethidium bromide and UV
-Computerized gel-imaging

SPECIAL EQUIPMENT
-Gibco Enzymes
-Fotodyne Gel Imaging System

Sarah Crosky's RFLP diversity data.

Sarah Crosky and Peter Williams load agarose gels (April, 2001).