Detection of Human DNA Mutations using Base Excision Sequence Scanning (BESS) and the LI-COR IR2 Automated DNA Sequencer

Harry Osterman and Gi Y. Jang - LI-COR Inc., Biotechnology Division, Lincoln, NE 68504

Summary

The combination of the BESS T-scan™ method with the IR2 system allows both strands of a PCR product to be scanned quickly for sequence variation. The dUTP is incorporated during PCR and the cleavage reaction requires only a 15-30 minute incubation following which samples can be directly analyzed. This method offers a rapid approach to screening populations for a variety of sequence variation including SNPs.

Acknowledgement

This work was done in collabaoration with Greg Hawkins at Epicentre Technologies who provided reagents and samples.

References

1) Hawkins , G.A. et. al, Nature Biotechnology 15, 803, (1997).

2) Jang, G. and Osterman, H., Epicenter Forum, 4, 1 (1997).

3) Anderson, S., et al. Nature 290, 457 (1981).

 

BESS T-scan is a trademark of Epicentre Technologies. Patent Pending, Epicentre Technologies.

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