Biotechnology
| Poster #520 | |
| » | Abstract |
| » | Introduction |
| » | Materials and Methods |
| » | Results and Discussion |
| » | Conclusions |
| » | Acknowledgements and References |
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Bovine Microsatellite Multiplexing for Herd
Evaluation and Parentage
J. Kovar, J. Walker, D. Steffens, J. Harford, and J. Qiu
LI-COR Inc., 4647 Superior Street, Lincoln, NE 68504, USA.
Abstract
Confirmation of parentage and/or typing of a specific group of cattle may be necessary when considering herd improvements, registry, and breed association. We have designed a protocol that allows for the detection of 10 bovine microsatellites (recommended by the International Society of Animal Geneticists and American Breeders Society) that exhibit a high level of heterozygosity to facilitate tracking of alleles through a population. By incorporating the two IRD-label system (IRD700 and 800) within one PCR reaction, one can increase the number of microsatellites evaluated per reaction. Therefore, by organizing all ten bovine microsatellites in question into two multiplex PCR panels we are able to achieve an increase in efficiency and throughput on the NEN® Global IR2 System. The production of a specific molecular weight marker system for each panel facilitates the scoring of alleles and their analysis with the automated program, SAGA GT™.
