Dyes for Your Near-Infrared Imaging Needs

IRDye® 650 Infrared Dye

IRDye 650 has a maximum absorbance at 648 nm and a maximum excitation at 665 nm. This dye is available as an NHS ester or maleimide form.

IRDye 650 absorption and emission spectra in methanol. Absorbance max - 648 nm; emission max = 665 nm.
Solvent Ext. Coeff. (M-1cm-1) Abs Max (nm) Em Max (nm)
Methanol 240,000 651 668
1X PBS 230,000 648 665
PBS: Methanol 230,000

NHS Ester

IRDye 650 NHS Ester is an infrared dye with detection near 650 nm. The NHS ester reactive group provides the functionality for labeling primary amino groups.


Maleimide

IRDye 650 Maleimide is an infrared dye with detection near 650 nm. Maleimide reactive group provides the functionality for labeling molecules that contain free sulfhydryl (-SH thiol) groups. This reactive group allows conjugations reactions to be performed at physiological pH.


 

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