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Dyes for Your Near-Infrared Imaging Needs
IRDye® 700DX Infrared Dye
The phthalocyanine dye IRDye 700DX has robust properties that make it suitable for optical imaging when conjugated to antibodies, receptor-binding ligands, small molecules, and more. It is considerably less sensitive to photobleaching than many other organic fluorochromes, and has excellent water solubility and salt tolerance.
Features of IRDye 700DX:
NHS ester reactive group for labeling primary and secondary amino groups
Dye of choice for protein, antibody, or nucleic acid labeling applications where high water solubility is essential
Potential as a molecular-targeted cancer therapy, or photoimmunotherapy (PIT)1
Excellent photostability, water solubility, and salt tolerance2
Alternative to Quantum Dots
1. M. Mitsunaga, M. Ogawa, N. Kosaka, L.T. Rosenblum, P.L. Choyke, H. Kobayashi, Cancer cell-selective in vivo near infrared photoimmunotherapy targeting specific membrane molecules. Nature Medicine17, 1685-1691, 2011.
2. X. Peng, D.R. Draney, W.M. Volcheck, G.R. Bashford, D.T. Lamb, D.L. Grone, Y.Zhang, C.M. Johnson, Phthalocyanine dye as an extremely photostable and highly fluorescent near-infrared labeling reagent. Proceedings of SPIE 6097, 2006.
High Photostability of IRDye® 700DX
Photostability was tested by exposure to many sequential rounds of laser imaging.
Remains fluorescent after extended laser exposure
More photostable than other 700 nm dyes, including Alexa Fluor 680
[LEFT] Equimolar amounts of each dye (25 fmol) were spotted onto nitrocellulose membrane. The membrane was scanned repeatedly with the Odyssey Infrared Imaging System, and signal intensity was normalized to maximum intensity at time = 0.
Substantially more photostable than tetramethylrhodamine (TMR), one of the most photostable fluorescent dyes.1
Table 1. Photostability of IRDye 700DX, compared to other near-IR fluorescent dyes, and the very photostable TMR dye
| Dye | Irradiation intensity I (W/cm2) | Intensity Gain factor at surface (Gi) | Absorption cross section σ01 (λ) (cm2) | Excitation rate constant k01 (λ) (excitation /sec) | Measured fluorescence decay rate kz (bleached dye/sec) | Mean number of survived excitation cycles µ |
|---|---|---|---|---|---|---|
| IRDye 700DX | 24.711 | 2.823 | 6.200E-16 (680 nm) | 1.479E+05 (680 nm) | 0.024 | >6.161E+6 |
| Alexa Fluor 680 | 4.961 | 2.823 | 5.355E-16 (680 nm) | 2.564E+04 (680 nm) | 0.535 | 4.79E+04 |
| Cy5.5 | 8.790 | 2.823 | 7.270E-16 (680 nm) | 6.168E+04 (680 nm) | >0.454 | 1.36E+05 |
| Cy7 | 27.449 | 2.823 | 3.440E-16 (780 nm) | 1.045E+05 (780 nm) | 0.794 | 1.317E+05 |
| IRDye 800CW | 17.475 | 2.823 | 9.180E-16 (780nm) | 1.776E+05 (780 nm) | 1.730 | 1.027E+05 |
| TMR | 25.073 | 2.823 | 2.340E-16 (531 nm) | 4.422E+04 (531 nm) | 0.191 | 2.32E+05 |
1. X. Peng, D.R. Draney, W.M. Volcheck, G.R. Bashford, D.T. Lamb, D.L. Grone, Y.Zhang, C.M. Johnson, Phthalocyanine dye as an extremely photostable and highly fluorescent near-infrared labeling reagent., Proceedings of SPIE 6097, 2006.
