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MPX™ BLOTTING SYSTEM
Screen Multiple Samples + Targets on the Same Blot
MPX™ (Multiplexer) Blotting System
Screen multiple samples and multiple targets all on the same blot. No cutting. No stripping. No cross-contamination. Adapt your existing Western blotting procedure for comprehensive antibody screening with the MPX Blotting System.
Ideal for any multiple-target Western blot application, the MPX increases throughput, maximizes efficiency and reduces relative overall cost. Quantitative analysis is possible with utilization of the Odyssey® Imaging Systems and Odyssey system-optimized reagents.
- Click here to download the MPX Blotting User Guide
(This document is only available online.)
Ordering Information
- 921-00000 MPX Blotting System
- 921-00110 MPX Clamp Nut
- 921-00120 MPX Membrane Cushion
- 921-00200 Single Marker/ One Lane Comb
- 921-00202 Single Marker / Two Lane Comb
- 921-00204 Single Marker / Three Lane Comb
- 921-00206 Single Marker / Four Lane Comb
[LEFT] Representative MPX Western Blot with one sample and multiple targets
Application Versatility
Technical Note:
One blot Western optimization using the MPX™ Blotting System
LI-COR Biosciences
The MPX can be incorporated into a variety of assays. Examples include: primary and secondary antibody screening, monoclonal antibody screening, signal transduction pathway analysis, and time course studies. However, it is possible to adapt nearly all common Western procedures that generate a PVDF or Nitrocellulose blot of 7 x 8.5 cm to the MPX format.
Simple, Convenient Workflow
Channel ports are conveniently spaced, staggered and beveled creating a simple workflow for using both standard and multi-channel pipettes. The low volume channel ports accommodate a maximum volume of 160 µl, thus conserving precious and costly antibody. The nesting dovetail joint design makes it easy to handle multiple-blot processing by allowing the continuous linkage of units.
Screen Up To 48 Targets on One Blot
Processing is as simple as clamping the blot into the MPX creating up to 24 independent channels. The range of usable channels per sample is relative to comb-size---from 24 on a single sample to four on a four-sample blot. Using LI-COR’s selection of IRDye® infrared dyes on the Odyssey Imagers provides a maximum of 48-targets on a single membrane— two per channel—and the option for quantitative analysis. Other detection methods, such as standard chemiluminescence and colormetrc methods can provide a maximum 24 targets.
Compatibility
The MPX is compatible with all common Western blotting procedures that generate a nitrocellulose or PVDF blot of 7 cm x 8.5 cm. It can be utilized with all standard chemiluminescence, colorimetric, and infrared protocols. The channel ports are spaced to accommodate most common multichannel pipettes.
MPX vs Standard Western
Blot Cost Comparison Using Near-Infrared Detection |
Reagents |
IR
Detection 2-targets (15-well gel) |
Chemiluminescence (15-well gel) |
24-well MPX IR Detection |
|
1st
-target |
2nd-target |
|||
| Blocking Buffer | 7.80 |
7.80 |
7.80 |
5.20 |
| Primary Antibody ß-Tubulin (LI-COR, 926-4211) $239 (100µL) ERK 1 (Santa Cruz SC-94) $229 (200 µg/mL,200µL) |
23.56 18.32 |
23.56 --- |
--- 18.32 |
4.71 1.04 |
| Secondary Antibody (Recommended Dilutions: 1:15,000 for IR, 1:25,000 for Chemi, 1:5,000 for MPX) |
0.82 |
0.09 |
0.09 |
0.08 |
| Chemiluminescent Substrate (0.1 ml/cm2) |
0.00 |
12.20 |
12.20 |
0.00 |
| Film (2 sheets/blot) | 0.00 |
7.71 |
7.71 |
0.00 |
| Protein Markers (Recommended Amount: 1µL for IR; 10 µL for Chemi) |
0.22 |
2.26 |
2.26 |
0.22 |
| Total ($) | 50.72 |
52.62 |
48.38 |
11.25 |
| $ per Data Point | 1.69 |
3.51 |
3.23 |
0.23 |
Training Video
Below is a short demonstration showing the suggested use of the MPX Blotting System. The video shows proper layout of the membrane, addition of antibodies by both single channel and multichannel pipettes, and tips and tricks for getting optimum results.
Manual and Data Sheet
Application Notes and Protocols
Poster
Specifications
MPX Blotting System
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MPX Combs
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