ODYSSEY MousePOD®

One Probe Simplifies Discovery

The MousePOD^® in vivo Imaging Accessory allows you to easily convert your Odyssey Infrared Imaging System into an in vivo imager. Now, the Odyssey System can be your complete solution for in vitro, in vivo, and ex vivo analysis – all on a single, affordable platform.

Odyssey MousePOD

• Snaps into place over Odyssey^® scanning surface
• Install and remove as needed
• Accommodates up to 3 mice
• Includes in vivo imaging tools for automatically marketing tumors, organs, and other regions of interest
• Inlet and Outlet ports for gas anesthesia
• an Anesthesia System (9300-100) and Rotameter (926-72300) can be purchased separately, if needed
• Label your own probe, or try our ready-to-use IRDye imaging agents

Near-infrared Dye Probes Simplify Methodology and Discovery

One Probe Simplifies Discovery

Use the Odyssey system and IRDye^® near-infrared dyes for all the steps in your molecular imaging workflow. Performing all your studies with the same probe and fluorescent label saves time, and makes data interpretation more consistent.

• Cell-based assays

• Test the binding and specificity of your probe with in vitro cell-based assays, before you begin animal work
• Quick, convenient, and cost-effective method

• Whole animal imaging

• Use the MousePOD accessory to image anesthetized, intact animals and view distribution of the fluorescent probe

• Ex vivo tissue analysis and histology

• Analyze and quantify fluorescent signal in excised tissues or organs, or tissue sections, to determine distribution of the probe

• Simultaneous two-color fluorescence detection

• Flexible imager can perform many applications, including quantitative Western blotting

• IRDye optical probes can also be visualized by fluorescent microscopy

Workflow

Figure 1. Simplified workflow for Optical Probe Development.  For more detailed discussion, see
Kovar, J et al. A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models. Anal Biochem. 367(1):1-12 (2007)

Webinars

• Why Image in the NIR?

• "Advances in In Vivo Imaging: Near-Infrared Optical Imaging of Mice" Jeff Harford, LI-COR

• “Instrumentation and Imaging Considerations” Eva Sevick-Muraca, Baylor College of Medicine

• “In vivo imaging of prostate cancer using an IRDye 800CW EGF Optical Probe" Melanie Simpson, University of Nebraska – Lincoln

• “Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer” Eva Sevick-Muraca and Shi Ke, Baylor College of Medicine

• Development of imaging agents

• “Systematic Evaluation and Use of Targeted IRDye labeled Optical Contrast Agents” Mike Olive, LI-COR

• “Systematic Evaluation of Targeted IRDye Labeled Optical Imaging Agents” Joy Kovar, LI-COR

• “Near Infrared Fluorescent Approaches to Cell-Based Assays and Small Animal Imaging” Amy Geschwender, LI-COR

• Targeted imaging

• "Illuminating Prostate Cancer Progression" Melanie Simpson, University of Nebraska - Eppley Cancer Center

• "IRDye^® 800 2DG: A Broadly Applicable Tumor Imaging Agent for Preclinical Research" Mike Olive, LI-COR

• Five-Part Educational Webinar Series: Optical Imaging

• "Part 1: A Technology Introduction to Optical Imaging"
  Jeff Harford, Senior Product Manager, LI-COR

• "Part 2: A Technical Overview of in vivo Optical Imaging Agents & Targets”
  Peter Johnson, Senior Field Applications Scientist, LI-COR

• "Part 3: A Technical Overview of Optical Imaging: Light Sources & Target Illumination"
  Rex Peterson, Engineering Product Support Manager, LI-COR

• "Part 4: A Technical Overview of Optical Imaging: Dye Absorption & Fluorescence"
  Rex Peterson, Engineering Product Support Manager, LI-COR

• "Part 5: A Technical Overview of Optical Imaging: Light Collection, Imaging Display & Dynamic Range"
  Rex Peterson, Engineering Product Support Manager, LI-COR

Publications

Publishing with LI-COR data?

• Rosenzweig, HL et al.
  Activation of nucleotide oligomerization domain 2 exacerbates a murine model of proteoglycan-induced arthritis.
  J Leukoc Biol. 85(4):711-8 (2009)

• Duysen, EG and Lockridge, O.
  Whole body and tissue imaging of the butyrylcholinesterase knockout mouse injected with near infrared dye labeled butyrylcholinesterase.
  Chem Biol Interact. 175(1-3):119-24 (2008)

• Senf, SM et al.
  Hsp70 overexpression inhibits NF-kappaB and Foxo3a transcriptional activities and prevents skeletal muscle atrophy.
  FASEB J. 22(11):3836-45 (2008)

• He, X et al.
  Cytotoxicity of Paclitaxel in Biodegradable Self-Assembled Core-Shell Poly (Lactide-Co-Glycolide Ethylene Oxide Fumarate) Nanoparticles.
  Pharm Res. 25(7):1552-62 (2008)