4300 ANALYZER
Reliability to meet your Research and Educational Needs
For the professional, student, or faculty researcher,
the 4300 DNA Analyzer from LI-COR will accelerate your research and improve the quality of your data
High confidence in more precise data is the edge that researchers using LI-COR DNA analyzer have had for more than 20 years.
The 4300 System is a third generation instrument based on LI-COR's highly sensitive infrared fluorescence detection technology.
Overview & Advantages
Infrared Advantage
4300 instrumentation, software, reagents, and applications are all part of an integrated system designed to achieve the highest accuracy. Infrared detection is a key advantage since biological molecules, gels, and glass plates have little infrared fluorescence. With low background fluorescence, dye signals are clean and strong. Infrared detection gives 4300 DNA Analyzers excellent sensitivity.
Independent Detection
4300 DNA Analyzers feature LI-COR's unique, two channel detection that eliminates errors due to fluorescence overlap. The fluorescence of the two IR dyes are separated by 100 nm. Two separate sets of optically tuned lasers and detectors measure fluorescence from IR dyes independently.
High Sensitivity and Wide Dynamic Range
Underloaded or overloaded samples are accurately detected because LI-COR images use more than 65,000 grayscales per pixel (16-bit) to display the wide dynamic range of the 4300 DNA Analyzer. Unprocessed fluorescence data are captured in TIFF image format for highest fidelity.
Rugged and Reliable
Two Highly Reliable Diode Lasers
The 4300 DNA Analyzer is designed for multi-purpose operation. In addition to being an excellent research tool, the 4300 DNA Analyzer is designed to meet demanding undergraduate training and research environments. Features such as diode lasers (similar to a CD player) make the instrument rugged and dramatically reduce maintenance and operation expenses. The instrument can also operate on demand after periods of inactivity without any expensive start-up costs.
Two Separate Detectors
As samples pass in front of the scanning laser/microscope, two solid-state avalanche photodiodes detect fluorescence. Optical filtering assures that each detector measures fluorescence from only one of the infrared dyes.
Two Real Time Images
A separate image (similar to an autoradiogram) for each IR dye is collected in real time and can be displayed in an Internet browser or LI-COR application software (e-Seq, Saga, etc.).
Easily Adapts to any Lab or Network
The 4300 instrument contains a server that can operate either in a stand-alone configuration or connected to your network. An Internet browser or LI-COR Application Software can be used to start electrophoresis. The server software completely controls electrophoresis and data storage during a run. Two 16-bit TIFF images are stored on the 4300's server during a run. The 4300 System gives you maximum data access. Any computer can connect to the 4300 System via Internet browser or LI-COR Application Software (e-Seq, Saga, etc.). In a network environment, image data can be accessed from your office or over the Internet. Secure, password-protected server software assures that only members of your project have access to your data. The 4300 System is as expandable as the Internet. You can add all the capacity you'll ever need, without ever buying another computer. One computer can connect to any number of 4300 Systems to start runs or retrieve and analyze data.
Software
AlignIR™
AlignIR™ provides sequence alignment and assembly functions. AlignIR can operate as an independent program or as an add-on to e-Seq software that automatically assembles or aligns sequence data after autoseqeuncing.
e-Seq™
e-Seq is LI-COR's Windows-based DNA sequencing software that maximizes both connectivity and sequencing automation. e-Seq controls electrophoresis, sequences electrophoresis image files, produces reports for database submission, and makes it easy to connect the 4300 System to your network.
Saga MX
With SagaMX AFLP® Software, there is nothing to do after sample loading but wait for gels to be automatically scored and added to the Oracle® database. Runs are started from Saga client software, after which the DNA Analyzer starts electrophoresis and controls data collection. After electrophoresis, Saga automatically transfers image data in autoradiogram format to the Saga Application Server for analysis and storage.
Saga GT
For microsatellite research, the 4300 System features SagaGT, the most advanced genotyping automation software available. SagaGT automates electrophoresis, lane finding, location of standards, calibration of band sizes, and allele scoring.
SagaGT is second generation software developed by LI-COR. Saga 1.0 (Unix) was initially developed at the University of Washington and licensed to LI-COR.
Support
Brochures
Download these brochures in PDF format
- Automated DNA Sequencing
- AFLP® Analysis
- SNP Discovery (Ecotilling)
- Automated Microsatellite Analysis
- Automated Reverse Genetics (Tilling®)
- Genomics Solutions for Educators
- 4300 Data Analysis System (Overview)
Manuals
Download all 4300 and related product manuals in PDF format here
Protocols
Download all 4300 related protocols in PDF format here
Applications for the 4300 DNA Analyzer
More Applications
LI-COR DNA Analyzers are versatile imaging systems capable of a variety of applications.
Here is a list of some of them with key publications.
Publications
LI-COR DNA Analyzers have been used for genomics research for more than 18 years and are cited in thousands of publications. For a sampling of some of the research done, click on the link below.
Publications
Find 4300 related publications here
4300 Specifications
Laser/Microscope
Laser Lifetime: 40000 - 60000 hours typical.
700 Channel Laser Source: Solid-state diode laser at 685 nm.
800 Channel Laser Source: Solid-state diode laser at 785 nm.
Detectors: Two silicon avalanche photodiodes.
Scanning Speed: Five selectable scan speeds to optimize image quality.
Electrophoresis Power Supply
Maximum Output: 5000V, 40mA, and 120W.
Operation Specifications
Operating Conditions: 15 - 35C and dew point not greater than 19 C.
Power Requirements: Automatic voltage selection at 90-250 VAC and 47-63 Hz. 3 amps at 120volts; 380 watts maximum.
Dimensions
4300S: 59H x 51W x 47D cm (23" x 20"x 18"). 102 cm (40") wide with door fully open.
4300L: 84H x 51W x 47D cm (33" x 20"x 18"). 102 cm (40") wide with door fully open.
Weight
4300S: 29 kg (64 lb).
4300L: 36.3 kg (80 lb).
Internal Server
Data Storage Capacity: 80 GB for data storage and server software.
Network Protocol: TCP/IP.
Network Connection: RJ-45, 10/100 BaseT.
Security: Password protected access.
Gel Electrophoresis Apparatus
| Glass Height | Separation Distance | Resolution (bp) | Run Time (h) | Applications |
|---|---|---|---|---|
| 66 cm* | 56 cm | 1000-1200 | 10 | Sequencing |
| 41 cm | 31 cm | 800 | 6 | Sequencing |
| 25 cm | 15 cm | 500 | 1.5 - 3 | Genotyping and Sequencing |
