AERIUS^® System Combines the Infrared Advantages
of the Odyssey Infrared Imaging System with Automation for Plate-Based Assays

The Aerius Automated Imaging System provides you with extreme sensitivity and quantitative analysis of proteins with a fast and efficient microplate reader.

In addition to processing up to 30 microplates per run for large-scale analysis, the Aerius System can be used to scan single membranes, gels, or microplates for validating antibodies.

Aerius^® Scanning Features

• Two-color scanning for simultaneous detection of two protein targets or the use of one channel for normalization.

• 20 – 500 µm resolution.

• Scans standard microplates

• Scans membranes or gels with the Aerius Membrane Carrier

• Integrate scanning and automatic analysis for In-Cell Western™ assays.

• Simple operation - scanning and In-Cell Western assay analysis are reduced to choosing an express run template.

Purchase your system with or without the stacker for automation.

Aerius Automated Infrared Imaging System Brochure

AERIUS^® Scanning Features

• Two-color scanning for simultaneous detection of two protein targets or the use of one channel for normalization.
• 20 – 500 µm resolution.
• Scans standard microplates or membranes using the Aerius Membrane Carrier.
• Integration of scanning and automatic analysis for In-Cell Western^™ assays.
• Simple operation. Scanning and In-Cell Western analysis are reduced to choosing an express run template.

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AERIUS^® Automation Features

User choice of fully automated or interactive analysis of scanned images.

Bio-Tek^® Bio-Stack^™ Automated Microplate Stacking System

• Aerius Express run software integrates with the plate stacker to serially scan up to 30 microplates per run.
• Express run templates allow you to save configurations for an entire stack of plates.
• During a run, each plate is scanned, analyzed with In-Cell Western analysis software, and data output in reports or data files.
• Automatic output to a network location in delimited text format for spreadsheets, laboratory information systems, or analysis software.

For repetitive runs, scanning is as simple as choosing an express run template that defines scanning and analysis for up to 30 microplates.

Applications for the AERIUS

Aerius introduces a new level of automation for In-Cell Western^™ assays and an expanding list of other infrared plate-based assays. Aerius provides fully automated data analysis of In-Cell Westerns for true, one-touch, walk-away automation.

In-Cell Western™ Assay On-Cell Western Assay Protease Assay Quantitative Western Blot RNAi Screens Transcription Factor Assay

Aerius User Guide

Aerius Applications Protocols Manual

Publications & Webinars

Publishing with LI-COR data?

Peng, X et al.
A nonfluorescent, broad range quencher dye for Förster resonance energy transfer assays
Anal Biochem. 388(2):220-28 (2009)

Kovar, J et al.
Characterization and performance of a near-infrared 2-deoxyglucose optical imaging agent for mouse cancer models
Anal Biochem.384(2): 254-62 (2009)

Kovar, J et al.
Purification method directly influences effectiveness of an epidermal growth factor-coupled targeting agent for noninvasive tumor detection in mice
Anal Biochem. 361(1):47-54 (2007)

Kovar, J et al.
Hyaluronidase expression induces prostate tumor metastasis in an orthotopic mouse model
Am J Pathol. 169(4):1415-26 (2006)
Used to lock the Bio-Tek^® Bio-Stack^™ Automated Plate Stacking System in position at the correct height and distance from the Aerius Imager (9250-01) and includes communication cables to connect the two instruments.

Accessories

Ordering Information:

• Membrane Carrier

• Used for Western blotting methods and other analyses requiring scans of membranes. Carrier has the same dimensions as a microplate, but with a 3 mm ridge around the edges to prevent liquid from spilling into the Aerius Imager

• Plate Stacker Adapter Kit

• Used to lock the Bio-Tek^® Bio-Stack™ Automated Plate Stacking System in position at the correct height and distance from the Aerius Imager (9250-01) and includes communication cables to connect the two instruments.

• Calibration Ruler

• Replacement part for Plate Stacker Adapter Kit (9250-14)

• Odyssey Pens

• Used for writing on membranes to be scanned. Does not fluoresce in the 700 or 800 channel

Please see our new Online Catalog for the latest ordering information.

Aerius Specifications

• Laser/Microscope
  • Laser Lifetime: 40,000 hours typical
  • 700 Channel Laser Source: Solid-state diode laser at 685 nm
  • 800 Channel Laser Source: Solid-state diode laser at 785 nm
  • Detectors: Silicon avalanche photodiodes
  • Scanning Speed: 6 minutes per microplate with 100 µm resolution
  • Resolution: 20 - 500 µm
  • Focusing Range: 0 - 3.95 mm (suitable for most microtiter plates with transparent, flat-bottom wells.)


• Operation Specifications
  • Operating Conditions: 15-35° C and dew point no greater than 20° C
  • Power Requirements: Automatic voltage selection at 90-250 VAC and 47-63 Hz; 1.1 Amps at 120 V; 200 watts maximum
  • Dimensions (instrument only): 36 h x 45 w 57 d cm (14 x 18 x 22 inches)
  • Dimensions (instrument with stacker): 85 h x 110 w x 45 d cm (33 x 44 x 18 inches)
  • Weight: 26 kg (56 lbs)
  • Data Storage Capacity: > 25 GB
  • Network Protocol: TCP/IP
  • Network Connection: Cat. 5 RJ-45, 10 Base-T/100 Base-TX
  • Bar Code Reader symbology: Code 39 preferred. Compatiable with Code 128 and other symbologies.

Contact LI-COR for more information