Infrared Detection vs. Chemiluminescence

“LI-COR has brought down the costs of protein analysis by introducing an approach that takes advantage of the inherent sensitivity advantage of the near-infrared spectrum, thereby removing the need to develop film while performing immunoblots (a time-consuming, labor-intensive operation).

“Compared with traditional systems, the cost of performing an identical protein analysis experiment using the Odyssey system is 90% less. This system’s fluorescent detection and digital delivery of data improves its usefulness by providing quantitative numerical results, rather than prior methods that provided purely qualitative images.”

--Frost & Sullivan, September 25, 2006

Cost Comparison Table

Reagents	Typical Costs For A Single 10x10 Blot
	IR Detection (2 color)	Chemiluminescence (1 color)	Chemiluminescence (Strip and Reprobe)	Calculate Your Savings
Secondary Antibody Recommended Dilutions: 1:15000 for IR; 1:2500 for Chemi	$0.68	$0.32	$0.64	Enter your current costs below: $
Chemiluminescent Reagents (12mL)	$0.00	$7.41	$14.80	$
Film (2/4 Films/blot)	$0.00	$7.48	$14.96	$
Protein Markers Recommended Amount: 1uL for IR; 10uL for Chemi	$1.16	$4.68	$468	$
Cost:	$1.84	$19.89	$35.08	$ -
Extra Cost Per Blot*		$18.05	$33.24	$ -
			# of Blots/week	weekly savings... $ -
*Based on GE/Amersham pricing, May 23, 2008.
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