Multiplex Detection
Two-Color Detection and Quantification
Using fluorescent dye-labeled antibodies rather than an enzymatic reaction, detection using any of the Odyssey Imaging Systems offers a broad linear dynamic range to accurately detect strong and weak signals on the same Western blot.
Simultaneous detection of two targets on the same membrane increases the accuracy of quantification and comparison.
[Above] ERK1/2 and phospho-ERK were detected simultaneously in lysates of unstimulated and EGF-stimulated A431 cells. Two fold serial dilutions of lysate were loaded. Pseudocolor images can be overlaid (yellow indicates overlap of red and green). The mobility shift caused by phosphorylation can be seen in the EGF-stimulated lysate. Data obtained using the Odyssey Classic Imager.
