ODYSSEY IMAGER
Dr. Fazlul Sarkar, an innovative researcher in the cancer field for over 30 years, is now in his 19th year at the Karmanos Cancer Center at the Wayne State Medical School.
Upgrade with Confidence to Quantitative Westerns
In this free 14-minute video from LI-COR and Biocompare, LI-COR researcher Sally Weldon discusses:
- The benefits of near-infrared fluorescent detection
- Making the easy transition to Odyssey fluorescence
The Odyssey® Infrared Imaging System sweeps away the old paradigms of chemiluminescence and visible fluorescence...
and introduces a new standard for Western blot analysis –
direct infrared fluorescence detection.
Infrared detection gives you the quantitative analysis and wide linear dynamic range that chemiluminescence can’t. Strong and weak bands are accurately detected on the same blot without the uncertainty and inconvenience of multiple exposures or time in the darkroom.
Multiple applications are optimized for the Odyssey System.
Overview & Advantages
Wide Linear Range
Using fluorescently labeled antibodies rather than an enzymatic reaction, the Odyssey Imager offers a broad linear dynamic range to accurately detect strong and weak signals on the same Western blot.
Two-Color Detection and Quantification
Simultaneous detection of two targets on the same membrane increases the accuracy of quantification and comparison.
High Sensitivity
Near-infrared fluorescence provides equal or better sensitivity than chemiluminescence – giving you low background, high signal-to-noise, multiplexed detection, and the sensitivity of any fluorescent system.
The Infrared Advantage
Most fluorescent imaging systems use visible fluorescence.
Membranes and plastics can produce high background in the visible region, due to autofluorescence and light scatter.
This limits the sensitivity of visible fluorescence and makes it nearly impossible to detect low-abundance proteins.
The Odyssey uses near-infrared fluorescence, dramatically reducing autofluorescence and light scatter.
LI-COR Biosciences is the clear leader in fluorescent Western blot detection and near-infrared imaging, offering:
Unique family of IRDye® Infrared Dyes with reactive functionalities for easy coupling to antibodies and other biomolecules.
Wide selection of IRDye secondary antibodies, blocking buffers, and blotting membranes for fluorescent Western blot detection.
Compatibility of the Odyssey Imager with many other fluorescent reagents.
Direct Detection
With Odyssey system, there is no film, darkroom, plastic wrap, or substrates. IRDye signals on membranes are stable indefinitely if stored properly.
-
Flexible System with Many Applications
The Odyssey system has been used for many applications, including Western blot analysis, EMSA, protein arrays, In-Cell Western™ Assays, On-Cell Westerns, in vivo imaging, Coomassie gel documentation, DNA gel documentation and tissue section analysis.
Odyssey data have been widely published in the literature, in journals such as Science, Nature, PNAS, Journal of Cell Biology, Journal of Biological Chemistry, Cancer Research, PLos ONE, and many others.
[LEFT] ERK1/2 and phospho-ERK were detected simultaneously in lysates of unstimulated and EGF-stimulated A431 cells. Two fold serial dilutions of lysate were loaded. Psuedo-color images can be overlaid (yellow indicates overlap of red and green). The mobility shift caused by phosphorylation can be seen in the EGF-stimulated lysate.
[ABOVE] Two-fold serial dilutions of Alexa® 680-labeled antibody (6 ng to 0.19 pg) were spotted on nitrocellulose and imaged in the 700 nm channel.
Applications for the Odyssey
A wide-range of applications benefit from near-infrared detection on the Odyssey System
Our applications profies are designed to provide you with examples, support information, recent publications, and available on-line subject specific webinars.
Accessories for the Odyssey
Brochures and Papers
Webinars
POWER USER Guide
Tutorials for optimizing your work on the OdysseyOdyssey Infrared Imaging System from LI-COR Biosciences
- Sally WeldonQuantitative, Multiplexed Western Blot Detection with Infrared Fluorescence
- Amy Geschwender, PhDChemi IR™ Detection of Western Blots on the Odyssey Infrared Imager
- Shawn Mischnick
Odyssey® Publications, 6-2009 – 9/2009
Featured Publications from the Scientific Literature
Markovic, D et al.
Intracellular mechanisms regulating corticotropin-releasing hormone receptor-2beta endocytosis and interaction with extracellularly regulated kinase 1/2 and p38 mitogen-activated protein kinase signaling cascades.
Mol Endocrinol. 22(3): 689-706 (2008)Ramsay, AJ et al.
Kallikrein-related peptidase 4 (KLK4) initiates intracellular signaling via protease-activated receptors (PARs). KLK4 and PAR-2 are co-expressed during prostate cancer progression.
J Biol Chem. 283(18): 12293-304 (2008)Wang, YV et al.
Quantitative analyses reveal the importance of regulated Hdmx degradation for p53 activation.
Proc Natl Acad Sci USA. 104(30): 12365-70 (2007)Wishart, TM et al.
Differential proteomics analysis of synaptic proteins identifies potential cellular targets and protein mediators of synaptic neuroprotection conferred by the slow Wallerian degeneration (Wlds) gene.
Mol Cell Proteomics. 6(8): 1318-30 (2007)
References from HighWire Press®
The LI-COR Odyssey has been featured in hundreds of academic journals. The best, most up-to-date list can be found on this excellent search engine, HighWire Press, Internet Imprint of the Stanford University Libraries.
Use the links below to conduct find articles on the following topics:
References from Google Scholar™
Of course, Google sets the standard for internet searches. Many articles can be found with Google Scholar on the following topics:
OdysseySpecifications
Laser/Microscope
Laser Lifetime: 40,000 hours typical
700 Channel Laser Source: Solid-state diode laser at 680 nm
800 Channel Laser Source: Solid-state diode laser at 780 nm
Detectors: Silicon avalanche photodiodes
Scanning Speed: 5 - 40 cm/S
Resolution:21 - 337 µm
Focusing: Scan bed is movable in the Z-dimension, allowing the fluorescence detection microscope to be aligned to the top surface of the glass to obtain the best signal-to-noise ratio
Operation Specifications
Operating Conditions: 15-35° C and dew point no greater than 20° C
Power Requirements: Automatic voltage selection at 90-250 VAC and 47-63 Hz; 1.1 Amps at 120 V; 200 watts maximum
Dimensions: 37 h x 53 w 58 d cm (14.5 x 21 x 23 inches)
Weight: 33 kg (72 lbs)
Data Storage Capacity: 5 GB
Network Protocol: TCP/IP
Network Connection: Cat. 5 RJ-45, 10 Base-T/100 Base-TX
Security: Password protected access
