The Odyssey^® Infrared Imaging System -
The Quantitative Western Blot Standard for Almost 10 Years

Chemiluminescence and fluorescence detection methods are both commonly used in Western blot analyses. Near-infrared (NIR) fluorescence detection allows you to quantitate proteins over a much wider linear dynamic range than chemiluminescence can.

Strong and weak bands are accurately detected on the same blot without the uncertainty and inconvenience of multiple exposures or time in the darkroom. The signal is stable and can be detected today or months later.

Multiple applications are optimized for the Odyssey System.

We offer a selection of Odyssey Infrared Imaging packages. Select the optimal configuration for your laboratory's research needs.

I would like more information on the Odyssey Infrared System

Overview & Advantages

• Wide Linear Range

  Accurately detect strong and weak signals over a broad linear dynamic range.

• Two-Color Detection and Quantification

  Detect two targets simultaneously on the same membrane increases the accuracy of quantification and comparison.

• High Sensitivity

  Near-infrared fluorescence provides sensitivity equal or better than chemiluminescence giving you low background, high signal-to-noise, multiplexed detection, and the sensitivity of any fluorescent system.

• Direct Detection for Better Data and a Cleaner Planet

  No film, a darkroom, plastic wrap, or substrates. IRDye signals on membranes are stable indefinitely if stored properly.

• Flexible System with Many Applications

  The Odyssey system has been used for many applications, including Western blot analysis, EMSA, protein arrays, In-Cell Western™ Assays, On-Cell Westerns, in vivo imaging, Coomassie gel documentation, DNA gel documentation and tissue section analysis. 

• Buy from a Leader

  LI-COR Biosciences is the leader in fluorescent Western blot detection and near-infrared imaging.

Applications for the Odyssey^® Infrared Imaging System

A wide-range of applications benefit from near-infrared fluorescent detection on the Odyssey System

Our applications profiles are designed to provide you with examples, support information, recent publications, and available on-line subject-specific webinars.

Coomassie Gels DNA Gel Staining ELISA/FLISA EMSA/Gel Shift Assay Glycoprotein Detection In-Cell Western™ Assay In-Gel Western Assay Northern Blot On-Cell Western Assay Protease Assay

Protein Array Quantitative Western Blot Reporter Gene Assay Reverse Phase (Lysate) Array RNAi Screens Small Animal Imaging Southern Blot Tissue Section Imaging Transcription Factor Assay Microwestern

Related Products and Accessories for the Odyssey^® Infrared Imaging System

• Odyssey Pens

• Odyssey MousePOD in vivo Imaging Accessory

• Odyssey Blocking Buffer

• Odyssey Blocking Buffer & PVDF Membrane Kit

• Smart Gels

• Odyssey Two-Color Molecular Weight Markers (10-250 kDa)

• Blot Washer

• Western Blotting Kits

• MPX™ (Multiplexer) Blotting System

• Western Incubation Boxes

• NucleoCounter^® NC-100^™ for Automated Cell Counting

• Software

• Support Contracts

Brochures and Papers

• Odyssey^® Infrared Imaging System Brochure - UPDATED February 2010

• Molecular Imaging on the Odyssey Imager and MousePOD^®

• In-Cell Western™ Assays Brochure - UPDATED! April 2010

• IRDye® Brochure - NEW! April 2010

White Paper & Tools

• Quantitative, Two-Color Western Blot Detection With Infrared Fluorescence (White paper)

• IR vs. Chemi Cost Comparison Worksheet

Webinars

• POWER USER Guide
  Tutorials for optimizing your work on the Odyssey

• Odyssey Infrared Imaging System from LI-COR Biosciences
  - Sally Weldon

• Quantitative, Multiplexed Western Blot Detection with Infrared Fluorescence
  - Amy Geschwender, PhD

• Chemi IR™ Detection of Western Blots on the Odyssey Infrared Imager
  - Shawn Mischnick

[+] More Webinars

Publishing with LI-COR data?

Odyssey^® Publications, January 2010 - March 2010

Featured Publications from the Scientific Literature

• Markovic, D et al.
  Intracellular mechanisms regulating corticotropin-releasing hormone receptor-2beta endocytosis and interaction with extracellularly regulated kinase 1/2 and p38 mitogen-activated protein kinase signaling cascades.
  Mol Endocrinol. 22(3): 689-706 (2008)

• Ramsay, AJ et al.
  Kallikrein-related peptidase 4 (KLK4) initiates intracellular signaling via protease-activated receptors (PARs). KLK4 and PAR-2 are co-expressed during prostate cancer progression.
  J Biol Chem. 283(18): 12293-304 (2008)

• Wang, YV et al.
  Quantitative analyses reveal the importance of regulated Hdmx degradation for p53 activation.
  Proc Natl Acad Sci USA. 104(30): 12365-70 (2007)

• Wishart, TM et al.
  Differential proteomics analysis of synaptic proteins identifies potential cellular targets and protein mediators of synaptic neuroprotection conferred by the slow Wallerian degeneration (Wlds) gene.
  Mol Cell Proteomics. 6(8): 1318-30 (2007)

OdysseySpecifications

Laser/Microscope

• Laser Lifetime: 40,000 hours typical

• 700 Channel Laser Source: Solid-state diode laser at 680 nm

• 800 Channel Laser Source: Solid-state diode laser at 780 nm

• Detectors: Silicon avalanche photodiodes

• Scanning Speed: 5 - 40 cm/S

• Resolution:21 - 337 µm

• Focusing: Scan bed is movable in the Z-dimension, allowing the fluorescence detection microscope to be aligned to the top surface of the glass to obtain the best signal-to-noise ratio

Operation Specifications

• Operating Conditions: 15-35° C and dew point no greater than 20° C

• Power Requirements: Automatic voltage selection at 90-250 VAC and 47-63 Hz; 1.1 Amps at 120 V; 200 watts maximum

• Dimensions: 37 h x 53 w 58 d cm (14.5 x 21 x 23 inches)

• Weight: 33 kg (72 lbs)

• Data Storage Capacity: 5 GB

• Network Protocol: TCP/IP

• Network Connection: Cat. 5 RJ-45, 10 Base-T/100 Base-TX

• Security: Password protected access