Infrared Detection vs. Chemiluminescence

"LI-COR has brought down the costs of protein analysis by introducing an approach that takes advantage of the inherent sensitivity advantage of the near-infrared spectrum, thereby removing the need to develop film while performing immunoblots (a time-consuming, labor-intensive operation).

"Compared with traditional systems, the cost of performing an identical protein analysis, experiment using the Odyssey system is 90% less. This system's fluorescent detection and digital delivery of data improves its usefulness by providing quantitative numerical results, rather than prior methods that provided purely qualitative images."

-- Frost & Sullivan, September 25, 2006

Infrared Detection
Reagents Typical Costs for a Single 10cm x 10cm Blot
IR Detection (2 color) Chemiluminescence (1 color) Chemiluminescence (Strip and Reprobe) Calculate Your Savings
Secondary Antibody
recommended Dilutions:
1:15,000# for IR; 1:2500 for Chemi
$0.68 $0.33 $0.66
Chemiluminescent Reagents (12mL) $0.00 $5.70 $11.40
Film (2/4 Films/blot) $0.00 $7.68 $15.36
Protein Markers
Recommended Amount:
1uL for IR; 10uL for Chemi
$1.16 $4.68 $0.00
Cost: $1.84 $18.39 $27.42 -
Extra Cost Per Blot* $16.55 $25.58 -
# of Blots/Week Weekly Savings... -

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