Introducing the Odyssey Fc Imaging System

The Odyssey Fc Imager is the first CCD imager that offers excellent infrared (IR) fluorescent and chemiluminescent imaging performance in one system. The Odyssey Fc optical system combines solid-state laser diodes at 685 and 785 nm with a low-noise, CCD camera for detection of IR and chemiluminescent signals.

Chemiluminescence detection is simplified with the Odyssey Fc Imaging System. Film and darkroom steps are eliminated, along with the hazardous waste and excessive water usage of film development.

FieldBrite™ XT technology acquires images over the full dynamic range every time, with minimal user adjustments. With the proprietary technologies of the Odyssey Fc Imaging System, a single instrument can perform quantitative IR fluorescent detection PLUS qualitative chemiluminescent detection. Publication-quality images are easy to produce with either detection method. (Because chemiluminescence is a time-dependent enzymatic reaction, its quantitative range is limited. IR fluorescence is superior for quantification.)

The Odyssey Fc Imaging System includes LI-COR Image Studio Software for fast, easy image acquisition and analysis. At the push of a button, targets of interest can be imaged over more than six logs of linear dynamic range in every acquisition.

Overview & Advantages

• Wide Linear Dynamic Range

  Accurately detect strong and weak signals over a broad linear dynamic range.

• Two-Color Detection and Quantification

  Detect two targets simultaneously on the same membrane increases the accuracy of quantification and comparison.

• High Sensitivity

  Near-infrared fluorescence provides sensitivity equal or better than chemiluminescence giving you low background, high signal-to-noise, multiplexed detection, and the sensitivity of any fluorescent system.

• Image Studio Software

  User-friendly software with easy navigation makes acquisition and analysis straight-forward.

• Direct Detection for Better Data and a Cleaner Planet

  No film or darkroom needed. IRDye signals on membranes are stable indefinitely if stored properly.

• Flexible System with Many Applications

  The Odyssey Fc system has been used for many applications, including Western blot analysis, EMSA, In-Gel Westerns, Coomassie gel documentation and DNA gel documentation.

• Buy from a Leader

  LI-COR Biosciences is the leader in fluorescent Western blot detection and near-infrared imaging.

Applications for the Odyssey^® Fc Imaging System

Our applications profiles are designed to provide you with examples, support information, recent publications, and available on-line subject-specific webinars.

• Western Blotting

• Multiplex Westerns

• Gel Documentation

Related Products and Accessories
for the Odyssey^® Fc Infrared Imaging System

Odyssey Pens Odyssey Blocking Buffer Odyssey Blocking Buffer & PVDF Membrane Kit Smart™ Gels Odyssey Two-Color Molecular Weight Markers (10-250 kDa) Blot Washer

Western Blotting Kits MPX™ (Multiplexer) Blotting System Western Incubation Boxes NucleoCounter® NC-100™ for Automated Cell Counting Support Contracts

Brochures and Papers

• Odyssey^® Fc Infrared Imaging System Brochure - UPDATED June 2010

• IRDye® Brochure - NEW! April 2010

White Paper & Tools

• Quantitative, Two-Color Western Blot Detection With Infrared Fluorescence (White paper)

• Fluorescent Western Blotting (Biocompare article)

Webinars

• Quantitative, Multiplexed Western Blot Detection with Infrared Fluorescence
  - Amy Geschwender, PhD

[+] More Webinars

Publishing with LI-COR data?

Odyssey Fc Specifications

• Image Field Size: 10 cm x 12 cm

• Dynamic Range: 22 bit (>6 logs)

• Depth of Field for Best Sample Focus: 6 mm

• Patented FieldBrite™ XT Technology: CV <3%

• Laser Lifetime: 20,000 hours

• 700 Channel Laser Source: Solid-state Laser Diode at 685 nm

• 800 Channel Laser Source: Solid-state Laser Diode at 785 nm

• Detectors: Low-noise CCD. Thermoelectrically cooled.

• Acquisition Times:

  • Fluorescence (700 and 800 nm) channels: 30 s, 2 min, 10 min plus variable time feature
  • Chemiluminescence channel: 30 s, 2 min, 10 min, 60 min plus variable time adjustment feature

• Focusing: None needed

• Operating Conditions: 15-35°C and dewpoint no greater than 22°C, non-condensing

• Power Requirements: Universal input between 100-127 VAC (4 Amp) and 200-240 VAC (2 Amp); 50-60 Hz. Voltage fluctuations not to exceed 10% of the nominal voltage. Insulation Category II.

• Sensitivity: Similar to industry-leading Odyssey^® system.

• Dimensions: 41.4 cm W x 47 cm D x 67.3 cm H (16.3” W x 18.5” D x 26.5” H). Depth with imaging drawer open is 59.7 cm (23.5”).

• Weight: 27 kg (60 lb)

ETL/CETL/CE