The Pearl^® Imager

The Pearl^® Imager allows researchers to use native cells and cell lines for their in vivo imaging work. Laser excitation and optimized near-infrared fluorescence detection results in unparalleled sensitivity, as well as deep target imaging. Simple one-button operation and 30-second image acquisition make the Pearl Imager the preferred system in any core imaging lab.

The ready availability of leading targeting agents means your research can proceed immediately without have to wait for targeting agent development.

Overview & Advantages

• Excellent Sensitivity

  Laser based excitation and near-infrared optimization result in exceptional sensitivity with deeper tissue penetration and earlier target detection.
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• Fast Imaging

  One button operation and 30 second acquisition of 3 channels (700, 800 and white light) enables rapid screening of animals and simultaneous screening of different targets.
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• Native Cell Compatibility

  Using IRDye^® targeting agent solutions for fast research startup.
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• Enviromental Control

  With HEPA filtered imaging beds for immunocompromised animals, docking stations and anesthesia systems.
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• Pearl and Odyssey Imagers

  A complete Solution for targeting agent development and in vivo imaging.
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Applications for the Pearl Imaging System

• Small Animal Imaging
• Receptor Targeting
• Transporter Targeting
• Structural Imaging
• Biodistribution

Publications & Webinars

Publications

• Characterization and performance of a near-infrared 2-deoxyglucose optical imaging agent for mouse cancer models

• Hyaluronidase expression induces prostate tumor metastasis in an orthotopic mouse model

• Intrathecal delivery of fluorescent labeled butyrylcholinesterase to the brains of knock-out mice: Visualization and quantification of enzyme distribution in the brain

• RGD – Human serum albumin conjugates as efficient tumor Targeting Probes

• Purification method directly influences effectiveness of an epidermal growth factor-coupled targeting agent for noninvasive tumor detection in mice

• A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models

Webinars

Instrumentation and Imaging Considerations
- Dr. Eva M. Sevick-Muraca

Seminar on the value of NIR imaging and how it compares to other imaging technologies.

Advances in In Vivo Imaging: Near-Infrared Optical Imaging of Mice
- Jeff Harford,
LI-COR Biosciences

In Vivo Tracking of the BChE/IRDye^® 800CW Complex in BChE Knockout Mice
- Ellen Duysen

Seminar highlighting the use of the LI-COR Pearl Imager for detection of IRDye^® labeled BChE.

Systematic Evalutaion of Targeted IRDye^® Labeled Optical Imaging Agents
- Joy Kovar M.S.

A thorough discussion of optical agent development using the LI-COR Pearl^™ Imager.

Illuminating Prostate Cancer Progession
- Dr. Melanie Simpson

Utilization of the LI-COR Pearl Imager to measure the progression of prostate cancer in mice.

Specifications

• Dimensions: (WxDxH) cm: 41x41x64 (AND) Weight (lb/kg): ~55/25

• Dynamic Range: 22 bit

• Detector Type: CCD. Thermoelectrically cooled.

• Acquisition Time: 10-40s

• Resolution: 85 or 170 micron

• Capacity: 1 animal

• Normalize LUT: A single click links all selected images to the same look-up table for accurate visual comparison. (A look-up table converts fluorescent intensity values to pixel intensities.)

• Channels: 3

• Colors: 700 nm, 800 nm and white light

• Native Cell Compatibility: Yes. Does not require transfected cells or cell lines.

• Means of Targeting: Near-infrared labeled targeting agents.

• Custom Labeling: Via various near-infrared fluorescent dyes with NHS ester and maleimide functional groups and labeling kits.

• Standard Imaging Bed: 16.8 cm W x 12 cm D (6.6” W x 4.75” D). Vertical clearance to top of drawer is 3.8 cm (1.5”).

• Field of View: 11.2 cm W x 8.4 cm D (4.4” W x 3.3” D) at the surface of the imaging bed.

• Imaging Bed Temperature Range: 32-42 °C.

• Gas Anesthesia: Inlet and outlet ports flow anesthesia gas through a nose cone in the imaging drawer. A rotameter is included for controlling flow rate.

• Size: 41 W x 41 D x 66 cm H (16” W x 16” D x 26” H). Depth with imaging drawer open is 63.5 cm (25”). Portable. Small footprint for added mobility and easy placement in any lab.

• Weight: 23 kg. (50 lb).