Advancing Discovery with Infrared Imaging
Pearl Imaging System

Sensitive Infrared Detection for In Vivo Imaging

The Pearl® Impulse

Watch Pearl Imager Video

With the Pearl® Impulse, you can:

  • Visualize imaging agents dynamically with real-time imaging

  • Use native cells and cell lines for in vivo imaging, without reporter genes

  • Achieve exceptional sensitivity and deep target imaging with laser excitation and near-infrared fluorescent detection

  • Detect two near-infrared probes simultaneously

  • Acquire optimized images quickly with one-button operation

  • Use validated targeting agents to get started immediately without developing your own agents

Pearl Imager

Overview & Advantages

  • Excellent Sensitivity

    Laser-based excitation and near-infrared detection result in exceptional sensitivity, with deeper tissue penetration and earlier target detection.
    [Learn More]

  • Fast Imaging

    Rapid, one-button acquisition of 700 nm, 800 nm, and white light images enables quick screening of animals and simultaneous detection of different targets.
    [Learn More]

  • Native Cell Compatibility

    Using IRDye® targeting agent solutions for fast research startup – without reporter gene transfection or cell line development.
    [Learn More]

  • Enviromental Control

    With HEPA-filtered imaging beds for immunocompromised animals, docking stations and anesthesia systems.
    [Learn More]

Photon Illustrated

Photon Illustrated

To see Photon demonstrate
the advantages of near-infrared detection, click on the
Photon Illustrated link.

Applications for the Pearl Impulse Imager System

Publications

Publishing with LI-COR data?

Publications

Webinars

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Instrumentation and Imaging Considerations
- Dr. Eva M. Sevick-Muraca

Seminar on the value of NIR imaging and how it compares to other imaging technologies.

Watch Now
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Advances in In Vivo Imaging: Near-Infrared Optical Imaging of Mice
- Jeff Harford,
LI-COR Biosciences

Watch Now
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In Vivo Tracking of the BChE/IRDye® 800CW Complex in BChE Knockout Mice
- Ellen Duysen

Seminar highlighting the use of the LI-COR Pearl Imager for detection of IRDye® labeled BChE.

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Systematic Evalutaion of Targeted IRDye® Labeled Optical Imaging Agents
- Joy Kovar M.S.

A thorough discussion of optical agent development using the LI-COR Pearl Imager.

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Illuminating Prostate Cancer Progession
- Dr. Melanie Simpson

Utilization of the LI-COR Pearl Imager to measure the progression of prostate cancer in mice.

Watch Now

Specifications

  Pearl® Imager Pearl® Impulse
Detector Type CCD. Thermoelectrically cooled. CCD. Thermoelectrically cooled.
Scan Time Less than 30 seconds to complete white light, 700 nm channel and 800 nm channel image acquisition. Less than 30 seconds to complete white light, 700 nm channel and 800 nm channel image acquisition.
700 nm Channel Excitation 685nm, Emission 720nm Excitation 685nm, Emission 720nm
800 nm Channel Excitation 785nm, Emission 820nm Excitation 785nm, Emission 820nm
White Channel Excitation white, Emission, NA Excitation white, Emission, NA
Dyes IRDye® 680, IRDye 700DX, IRDye 800RS, IRDye 800CW, Alexa Fluor® 680, Alexa Fluor 750, Cy5.5, Cy7 IRDye® 680, IRDye 700DX, IRDye 800RS, IRDye 800CW, Alexa Fluor 680, Alexa Fluor 750, Cy5.5, Cy7
Resolution 85, 170 or 255 micron 85, 170 or 255 micron
Image Display Options Pseudocolor, grayscale, single color (red, green or blue), or two colors with overlapping fluorescence displayed in a third color. Pseudocolor, grayscale, single color (red, green or blue), or two colors with overlapping fluorescence displayed in a third color.
Capacity One animal, with linked look-up tables for image normalization One animal, with linked look-up tables for image normalization
Imaging Bed 16.8 cm W x 12 cm D (6.6” W x 4.75” D). Vertical clearance to top of drawer is 3.8 cm (1.5”). 16.8 cm W x 12 cm D (6.6” W x 4.75” D). Vertical clearance to top of drawer is 3.8 cm (1.5”).
Field of View 11.2 cm W x 8.4 cm D (4.4” W x 3.3” D) at surface of imaging bed. 11.2 cm W x 8.4 cm D (4.4” W x 3.3” D) at surface of imaging bed.
Imaging Bed Temperature Range 32-42 °C. 32-42 °C.
Gas Anesthesia Inlet and outlet ports flow anesthesia gas through a nose cone in the imaging drawer. A rotameter is included for flow rate control. Inlet and outlet ports flow anesthesia gas through a nose cone in the imaging drawer. A rotameter is included for flow rate control.
Size 41 W x 41 D x 66 cm H (16” W x 16” D x 26” H). Depth with imaging drawer open is 63.5 cm (25”). 41 W x 41 D x 66 cm H (16” W x 16” D x 26” H). Depth with imaging drawer open is 63.5 cm (25”).
Weight 23 kg (50 lb). 23 kg (50 lb).
Acquisition Speed ~30s 500 ms - 40s

Workstation

Build a Complete Solution

Combining the Odyssey® Infrared Imaging System with the Pearl® Impulse Imager creates a versatile workstation for in vivo and in vitro imaging. Probes developed using an IRDye® near-infrared dye can be used for evaluation of binding capacity in vitro, in vivo imaging and tissue imaging.

Complete solution

Odyssey® Imaging System Capabilities

  • Cell-based assays (binding capacity, specificity, competition, etc.) for optical agent development.

  • Histology and whole organ imaging for studying clearance and specificity.

  • Simultaneous two-color detection for two targets or one target with sample normalization.

Learn more about the workstation approach in optical agent development with this recent publication:


To see the workstation approach in action, download this free reprint:

 

 



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