The Pearl^® Impulse

With the Pearl^® Impulse, you can:

• Visualize imaging agents dynamically with real-time imaging

• Use native cells and cell lines for in vivo imaging, without reporter genes

• Achieve exceptional sensitivity and deep target imaging with laser excitation and near-infrared fluorescent detection

• Detect two near-infrared probes simultaneously

• Acquire optimized images quickly with one-button operation

• Use validated targeting agents to get started immediately without developing your own agents

Overview & Advantages

• Excellent Sensitivity

  Laser-based excitation and near-infrared detection result in exceptional sensitivity, with deeper tissue penetration and earlier target detection.
  [Learn More]

• Fast Imaging

  Rapid, one-button acquisition of 700 nm, 800 nm, and white light images enables quick screening of animals and simultaneous detection of different targets.
  [Learn More]

• Native Cell Compatibility

  Using IRDye® targeting agent solutions for fast research startup – without reporter gene transfection or cell line development.
  [Learn More]

• Enviromental Control

  With HEPA-filtered imaging beds for immunocompromised animals, docking stations and anesthesia systems.
  [Learn More]

Photon Illustrated

To see Photon demonstrate
the advantages of near-infrared detection, click on the
Photon Illustrated link.

Applications for the Pearl Impulse Imager System

• Small Animal Imaging
• Receptor Targeting
• Transporter Targeting
• Structural Imaging
• Biodistribution
• Vascular/Lymphatic Imaging

Publications

Publishing with LI-COR data?

Publications

• Ciallella, J. et al
  Near-Infrared Optical Imaging in Mouse Models of Parkinson's Disease, Alzheimer's Disease, and Contact Dermatitis
  Poster

• Kawase, T. et al.
  Human Periosteum-Derived Cells Combined With Superporous Hydroxyapatite Blocks Used as an Osteogenic Bone Substitute for Periodontal Regenerative Therapy: An Animal Implantation Study Using Nude Mice
  J. Periodont. 81(3):420-427

• Chen, Y et al.
  A Low Molecular Weight PSMA-based Fluorescent Imaging Agent for Cancer
  Biochemical and Biophysical Research Communications (390): 624-629 (2009)

• M. Q. XU et al.
  Study of Mouse Tumor Models with an IRDye 800CW SNAP-tag Conjugate
  Poster

• Bharadwaj, AG et al.
  Spontaneous metastasis of prostate cancer is promoted by excess hyaluronan synthesis and processing
  Am J Pathol. 174(3):1027-36. (2009)

• Johnson, ND et al.
  Intrathecal delivery of fluorescent labeled butyrylcholinesterase to the brains of knock-out mice: Visualization and quantification of enzyme distribution in the brain
  Neurotoxicology. 30(3): 386-92 (2009)

• Kovar, J et al.
  Characterization and performance of a near-infrared 2-deoxyglucose optical imaging agent for mouse cancer models
  Anal Biochem.384(2): 254-62 (2009)

• Chen, K et al.
  RGD – Human serum albumin conjugates as efficient tumor Targeting Probes
  Mol Imaging. 8(2):65-73 (2009)

• Banerjee, S et al.
  Antitumor activity of gemcitabine and oxaliplatin is augmented by thymoquinone in pancreatic cancer
  Cancer Res. 69(13):5575-83 (2009)

• Banerjee, S et al.
  3,3'-Diindolylmethane enhances chemosensitivity of multiple chemotherapeutic agents in pancreatic cancer
  Cancer Res. 69(13):5592-600 (2009)

• Kovar, J et al.
  Purification method directly influences effectiveness of an epidermal growth factor-coupled targeting agent for noninvasive tumor detection in mice
  Anal Biochem. 361(1):47-54 (2007)

• Kovar, J et al.
  A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models
  Anal Biochem. 367():1-12 (2007)

• Kovar, J et al.
  Hyaluronidase expression induces prostate tumor metastasis in an orthotopic mouse model
  Am J Pathol. 169(4):1415-26 (2006)

Webinars

Instrumentation and Imaging Considerations
- Dr. Eva M. Sevick-Muraca

Seminar on the value of NIR imaging and how it compares to other imaging technologies.

Advances in In Vivo Imaging: Near-Infrared Optical Imaging of Mice
- Jeff Harford,
LI-COR Biosciences

In Vivo Tracking of the BChE/IRDye^® 800CW Complex in BChE Knockout Mice
- Ellen Duysen

Seminar highlighting the use of the LI-COR Pearl Imager for detection of IRDye^® labeled BChE.

Systematic Evalutaion of Targeted IRDye^® Labeled Optical Imaging Agents
- Joy Kovar M.S.

A thorough discussion of optical agent development using the LI-COR Pearl^™ Imager.

Illuminating Prostate Cancer Progession
- Dr. Melanie Simpson

Utilization of the LI-COR Pearl Imager to measure the progression of prostate cancer in mice.

Specifications

Workstation

Build a Complete Solution

Combining the Odyssey^® Infrared Imaging System with the Pearl^® Impulse Imager creates a versatile workstation for in vivo and in vitro imaging. Probes developed using an IRDye^® near-infrared dye can be used for evaluation of binding capacity in vitro, in vivo imaging and tissue imaging.

Odyssey^® Imaging System Capabilities

• Cell-based assays (binding capacity, specificity, competition, etc.) for optical agent development.

• Histology and whole organ imaging for studying clearance and specificity.

• Simultaneous two-color detection for two targets or one target with sample normalization.

Learn more about the workstation approach in optical agent development with this recent publication:

• Kovar, J et al.
  A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models
  Anal Biochem. 367(1):1-12 (2007)

---

To see the workstation approach in action, download this free reprint:

• Kovar, J et al.
  Hyaluronidase expression induces prostate tumor metastasis in an orthotopic mouse model.
  Am J Pathol. 169(4):1415-26 (2006)