Chemi-IR™
Use your existing HRP secondary antibodies
Detection on the Odyssey® Infrared Imaging System
No substrates, film or darkroom
Familiar chemiluminescent methods made quantitative
Chemi-IR Western Blot
Detection of p53 in A431 Cell Lysate
[ABOVE] Serial dilutions of A431 lysate were run by SDS PAGE and transferred to nitrocellulose. The blot was probed with monoclonal anti-p53 followed by Donkey anti-mouse HRP. The blot was detected with IRDye 800CW Rabbit Anti-HRP.
[ABOVE] Each band of the Chemi-IR Western blot was quantified using Odyssey Imager analysis software. The graph shows the Odyssey detection method is linear to at least 78ng of A431 lysate.
Ordering Information:
Please see our new Online Catalog for the latest ordering information. Shipping information:
U.S.A.: Same-day shipping (Federal Express overnight) available on orders received before 12:00 noon CST.
Canada: Contact Mandel Scientific.
Germany & UK: Contact LI-COR GmbH.
International: Contact a LI-COR distributor in your area All shipments subject to availability.
Reagents |
Chemiluminescence |
Chemi-IR
with film & IR detection |
Chemi-IR
with IR detection only |
Protein Markers
Recommended Amount:
10µL for Chemi Detection |
$4.84 |
$4.84 |
$1.16 |
Anti-HRP Secondary Antibody
Recommended Dilution:
1:2500 |
$0.10 |
$0.10 |
$0.10 |
Chemiluminescent Reagents
(12mL) |
$12.97 |
$12.97 |
- |
Film
(2/4 Films/blot) |
$5.76 |
$5.76 |
- |
| Chemi-IR Detection |
- |
$4.00 |
$4.00 |
| Total Cost |
$23.67 |
$27.67 |
$5.26 |
Click here to view the Webinar presentation from May 19, 2009
