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Dyes for Your Near-Infrared Imaging Needs
IRDye® 800CW Secondary Antibodies
Optimized for Near-Infrared Fluorescent Detection
Cross-adsorbed for multiplex detection
Optimized for quantitative Western and In-Cell Western™ assays
High sensitivity and excellent signal-to-noise performance with LI-COR infrared imaging systems
Outperform visible fluorescent dyes
IRDye 680LT and IRDye 800CW conjugates
Achieve two-color detection by multiplexing IRDye 680LT and IRDye 800CW conjugates.
Click here to read more about quantitative Western blot detection with the Odyssey Classic, Odyssey Fc and Odyssey Sa Imaging Systems.
IRDye conjugates may be used for immunofluorescent microscopy and other fluorescent applications.
(requires appropriate light source and filters).
Subclass-specific secondary antibodies are also available. IRDye Goat anti-Mouse IgG1, Goat anti-Mouse IgG2a and Goat anti-Mouse IgG2b, allow for two-color detection using primary antibodies derived from the same species (mouse). IRDye Subclass Specific antibodies react with the heavy (gamma) chain only of the primary antibody. In mice, there are five unique subclasses of IgG; IgG1, IgG2a, IgG2b, IgG2c and IgG3. Each subclass is based on small differences in amino acid sequences in the constant region of the heavy chains so antibodies directed against a particular subclass will not recognize antibodies directed against other subclasses.
Optimized for a Wide Variety of Applications
IRDye infrared secondary antibodies are cross-adsorbed for multiplex detection and provide high specificity for any application. These antibody conjugates are optimized for high sensitivity and excellent signal-to-noise performance on LI-COR imaging systems, and outperform visible fluorescent dyes.
p-38 and Tubulin multiplex detection using infrared dye-labeled secondary antibodies. |
ERK and pERK multiplex detection using infrared dye-labeled secondary antibodies. |
Table 1. IRDye Infrared Dye Secondary Antibodies
| Specificity | Host | Form | IRDye® 800CW | IRDye® 680LT |
|---|---|---|---|---|
| anti-human IgG (H+L) | goat | lyophilized | 926-32232 (0.5 mg) | 926-68032 (0.5 mg) |
| anti-mouse IgG (H+L) | goat | lyophilized | 926-32210 (0.5 mg) | 926-68020 (0.5 mg) |
| anti-mouse IgG1 (H+L) | goat | lyophilized | 926-32350 (0.5 mg) | 926-68050 (0.5 mg) |
| anti-mouse IgG2a (H+L) | goat | lyophilized | 926-32351 (0.5 mg) | 926-68051 (0.5 mg) |
| anti-mouse IgG2b (H+L) | goat | lyophilized | 926-32352 (0.5 mg) | 926-68052 (0.5 mg) |
| anti-rabbit IgG (H+L) | goat | lyophilized | 926-32211 (0.5 mg) | 926-68021 (0.5 mg) |
| anti-mouse IgG (H+L) | donkey | lyophilized | 926-32212 (0.5 mg) | 926-68022 (0.5 mg) |
| anti-rabbit IgG (H+L) | donkey | lyophilized | 926-32213 (0.5 mg) | 926-68023 (0.5 mg) |
| anti-chicken IgG (H+L) | donkey | lyophilized | 926-32218 (0.5 mg) | 926-68028 (0.5 mg) |
| anti-rat IgG (H+L) | goat | lyophilized | 926-32219 (0.5 mg) | 926-68029 (0.5 mg) |
| anti-goat IgG (H+L) | donkey | lyophilized | 926-32214 (0.5 mg) | 926-68024 (0.5 mg) |
| anti-guinea pig IgG (H+L) | donkey | lyophilized | 926-32411 (0.5 mg) | 926-68030 (0.5 mg) |
*All conjugates are cross-adsorbed for minimal cross-reactivity.
