BrightSite™ IRDye^®
Small Animal Imaging Agents:

Near-infrared fluorescent probes for targeting of tumors, bone, lymphatics, and more.

• Validated with cell-based assays, microscopy, small animal imaging, and histology to ensure high affinity and specificity

• Simply administer the agent, then image the animal with a small animal imager equipped with an appropriate 680 nm and 800 nm filter set

• Compatible with most fluorescent small animal imaging systems, including instruments from LI-COR Biosciences, Caliper/CRi, Perkin Elmer/VisEn, and Carestream

BrightSite IRDye Small Animal Imaging Agents deliver:

• Sensitive detection in the animal – even for small or deep targets

• Ready-to-use simplicity

• Proven track record of peer-reviewed publications

BrightSite Agents include:

IRDye 800CW EGF IRDye 680RD EGF IRDye 800CW 2-DG IRDye 800CW RGD IRDye 680RD RGD

IRDye 800CW HA IRDye 680RD HA IRDye 800CW BoneTag™ IRDye 680RD BoneTag™ IRDye 800CW PEG

Brochure

• BrightSite Small Animal Imaging Agents Brochure

• BrightSite Small Animal Imaging Agents Data Sheet

• Pearl Impulse Brochure

Application Notes

• On-Cell Western Plate-Based Assay for Targeted Near-Infrared-Labeled Optical Imaging Agent Development: Receptor-Based Binding and Competition Assays

• Near-infrared-labeled tetracycline derivative is an effective marker of bone deposition in mice

Posters

• Kovar, J. L., et al.
  EGF-IRDye 800CW: in vitro and in vivo characterization as a biomarker for optical fluorescent imaging of tumor growth kinetics

• Kovar, J. L., et al.
  Monitoring progression of prostate tumors in mice by receptor-targeted near infrared optical imaging

• Ciallella, J. et al
  Near-Infrared Optical Imaging in Mouse Models of Parkinson's Disease, Alzheimer's Disease, and Contact Dermatitis

• Kovar, J.L., et al.
  GLUT Family Transporter Involvement in Cellular Update of IRDye^® 800CW 2-Deoxyglucose

• Kovar, J.L., et al.
  Integrin-specific near infrared optical imaging agent for tumor-induced angiogenesis detection in mice

• Kovar, J.L., et al.
  Effective Bone Labeling for In Vivo NIR Noninvasive Imaging in Nude Mice

Webinars

• Systematic Evaluation of Targeted IRDye^® Labeled Optical Imaging Agents
  - Joy Kovar, Sr. Scientist

• IRDye 800CW 2-DG: A Broadly Applicable Tumor Imaging Agent for Optical Preclinical Research
  - Dr. D. Michael Olive

• Cancer Research Webinar Series Part 5: Target Fluorescent Contrast Agents for Image-Guided Cancer Surgery
  - Dr. D. Michael Olive

• Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer
  - Dr. Eva Sevick-Muraca and Dr. Shi Ke, Baylor College of Medicine

• Cancer Research Webinar Series Part 3: In Vivo Imaging with Near-Infrared Fluorescent Optical Probes
  - Dr. Amy Geschwender

• More Small Animal in vivo Imaging Webinars

Publishing with LI-COR data?

• Spiotto, M. T., et al.
  Imaging the unfolded protein response in primary tumors reveals microenvironments with metabolic variations that predict tumor growth
  Cancer Res. 70: 78 (2010)

• Lowik, C. et al
  Optical Image-Guided Surgery - Where do we stand?
  Molecular imaging and Biology 2010. Doi: 10.1007/s11307-010-0373-2

• Gibbs-Strauss, SL et al.
  Detecting Epidermal Growth Factor Receptor Tumor Activity In Vivo During Cetuximab Therapy of Murine Gliomas
  Acad Radiol (17): 7-17 (2010)

• Zhao, D et al
  Dynamic Near-Infrared Optical Imaging of 2-Deoxyglucose Uptake by Intracranial Glioma of Athymic Mice
  PLoS ONE 4:e8051 (2009)

• Kovar, J et al
  Characterization and Performance of a Near-Infrared 2-Deoxyglucose Optical Imaging Agent for Mouse Cancer Models
  Anal Biochem. 384 (2009) 47-54.

• Bharadwaj, AG et al.
  Spontaneous metastasis of prostate cancer is promoted by excess hyaluronan synthesis and processing
  Am J Pathol 174(3):1027-36. (2009)

• Offterdinger, M et al.
  Prolonged EGFR Signaling by ERBB2-Mediated Sequestration at the Plasma Membrane
  Traffic 9(1):147-55 (2008)

• Adams, KE et al.
  Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer
  J Biomed Opt 12(2):024017 (2007)

• Kovar, J et al.
  Purification method directly influences effectiveness of an epidermal growth factor-coupled targeting agent for noninvasive tumor detection in mice
  Anal Biochem 361(1):47-54 (2007)

• Kovar, J et al.
  A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models
  Anal Biochem 367():1-12 (2007)

• Kovar, J et al.
  Hyaluronidase expression induces prostate tumor metastasis in an orthotopic mouse model
  Am J Pathol 169(4):1415-26 (2006)

• Kovar, J et al.
  Hyaluronidase Expression Induces Prostate Tumor Metastasis in an Orthotopic Mouse Model
  Am J Pathol 169:1415-1426 (2006)