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IRDye® 680LT Conjugates

Reagents for LI-COR Imaging Systems

"I just tried the sample anti-mouse IRDye 680LT, and it worked extremely well. The signal is as strong with exceptionally low background as we see in our 800/green channel. Thanks for bringing it to my attention and for the sample - we will switch to the new dyes immediately!"

- Sin Urban, Ph.D.
Associate Professor
Howard Hughes Medical Institute
Johns Hopkins University School of Medicine

IRDye 680LT Conjugates

New and Improved, Brighter 700 nm Conjugates

LI-COR IRDye infrared conjugates are optimized for a wide variety of applications, including Western blots, In-Cell Western™ Assays, In-Gel Westerns, On-Cell Westerns, protein arrays, tissue section imaging, and molecular imaging. All IRDye labeled secondary antibodies are highly cross-adsorbed.

IRDye 680LT secondary antibody comparison

[ABOVE] Serial dilutions of Jurkat lysate (10 µg/lane to 1.2 ng/lane) were resolved by SDS PAGE and transferred to nitrocellulose. Odyssey Blocking Buffer was used for all blocking and incubation steps. Blots were probed with Rabbit anti-p38 (Santa Cruz part no.sc-7149) diluted 1:1000 in Odyssey Blocker + 0.2% Tween 20 followed by detection with a 680 labeled antibody. All 680 labeled antibodies were diluted to a final concentration of 0.1 µg/ml in Odyssey Blocker + 0.2% Tween 20.

Product List Part#
IRDye 680LT Goat Anti-Human IgG (H + L), 0.5 mg 926-68032
IRDye 680LT Goat Anti-Mouse IgG (H + L), 0.5 mg 926-68020
IRDye 680LT Goat Anti-Mouse IgG1-Spacific, 0.5 mg 926-68050
IRDye 680LT Goat Anti-Mouse IgG2a-Spacific, 0.5 mg 926-68051
IRDye 680LT Goat Anti-Mouse IgG2b-Spacific, 0.5 mg 926-68052
IRDye 680LT Goat Anti-Mouse IgG (H + L), 25 µL 926-68020
IRDye 680LT Goat Anti-Rabbit IgG (H + L), 0.5 mg 926-68021
IRDye 680LT Goat Anti-Rabbit IgG (H + L), 25 µL 926-68020
IRDye 680LT Donkey Anti-Mouse IgG (H + L), 0.5 mg 926-68022
IRDye 680LT Donkey Anti-Rabbit IgG (H + L), 0.5 mg 926-68023
IRDye 680LT Donkey Anti-Goat IgG (H + L), 0.5 mg 926-68024
IRDye 680LT Donkey Anti-Chicken IgG (H + L), 0.5 mg 926-68028
IRDye 680LT Goat Anti-Rat IgG (H + L), 0.5 mg 926-68029
IRDye 680LT Donkey Anti-Guinea Pig IgG (H + L), 0.5 mg 926-68030
IRDye 680LT Streptavidin 926-68031

Ordering Information:
Please see our new Online Catalog for the latest ordering information.

 

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