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Reagents for LI-COR Imaging Systems
"I just tried the sample anti-mouse IRDye 680LT, and it worked extremely well. The signal is as strong with exceptionally low background as we see in our 800/green channel. Thanks for bringing it to my attention and for the sample - we will switch to the new dyes immediately!"
- Sin Urban, Ph.D.Includes:
New and Improved, Brighter 700 nm Conjugates
LI-COR IRDye infrared conjugates are optimized for a wide variety of applications, including Western blots, In-Cell Western™ Assays, In-Gel Westerns, On-Cell Westerns, protein arrays, tissue section imaging, and molecular imaging. All IRDye labeled secondary antibodies are highly cross-adsorbed.
[ABOVE] Serial dilutions of Jurkat lysate (10 µg/lane to 1.2 ng/lane) were resolved by SDS PAGE and transferred to nitrocellulose. Odyssey Blocking Buffer was used for all blocking and incubation steps. Blots were probed with Rabbit anti-p38 (Santa Cruz part no.sc-7149) diluted 1:1000 in Odyssey Blocker + 0.2% Tween 20 followed by detection with a 680 labeled antibody. All 680 labeled antibodies were diluted to a final concentration of 0.1 µg/ml in Odyssey Blocker + 0.2% Tween 20.
Ordering Information:
Please see our new Online Catalog for the latest ordering information.