IRDye^® 680LT NIR Fluorescent Dyes

The new IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes. The spectral characteristics of IRDye 680LT are well suited for use on LI-COR imaging instruments with absorbance and emission maxima in aqueous solution and methanol of 676 and 693 nm, respectively.

Dye Brightness
IRDye 680LT matches or surpasses the brightness of Alexa Fluor^® 680. Fluorescence determinations were made at a fixed antibody concentration of 10µg/mL in physiological buffer using dye-labeled goat anti-rabbit (GAR) conjugates prepared in-house. Fluorescence was measured using a PTI Fluorometer at the optimum excitation and emission wavelengths of each dye. The fluorescence intensity of each conjugate increased with increased degree of labeling. A plot of degree of labeling versus fluorescence (Figure 1) shows that the dynamic range for the 680LT GAR is much broader than the Alexa Fluor 680 which quickly levels off above D/P 3.4. The leveling off of the Alexa Fluor 680 conjugates is due to self-quenching. IRDye 680LT conjugates continue to increase in fluorescence intensity to at least D/P 6.4 which was the highest degree of labeling tested. Overall, IRDye 680LT is significantly brighter than Alexa Fluor 680.

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