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Dyes for Your Near-Infrared Imaging Needs
IRDye® 680RD EGF Optical Probe
Why Target EGFR?
IRDye 680RD EGF Optical Probe is a BrightSite™ near-infrared (NIR) labeled recombinant human epidermal growth factor (EGF). Epidermal growth factor receptor (EGFR) is one of a family of receptor tyrosine kinases found on the surface of epithelial cells, to which EGF binds. Many types of cancer cells have abnormally high EGFR levels on the cell surface (Table 1). In vivo assays of EGF binding have proven effective in monitoring specific solid tumor models that exhibit overexpression of EGFR. EGF conjugated to LI-COR's IRDye 680RD NIR fluorophore provides a versatile probe that can be used for in vitro and in vivo assays, as well as whole organ and tissue section analysis.
Figure 1. Intravenous administration of IRDye 680RD EGF (2 nmol) binds to the A431 tumor located on the right hip Image captured 24 h post injection on the Pearl® Impulse Imaging System.
Ordering Information
Please see our new Online Catalog for the latest ordering information.For research purposes only. Not for diagnostic or therapeutic use. Not for use in humans. LI-COR IRDye reagents are covered by U.S. patents, foreign equivalents, and patents pending.
Shipping information:U.S.A.: Same-day shipping (Federal Express overnight) available on orders received before 12:00 noon CST.
Canada: Contact Mandel Scientific.
Germany & UK: Contact LI-COR GmbH.
International: Contact a LI-COR distributor in your area All shipments subject to availability.
IRDye is a registered trademark of LI-COR Inc.
Advantages of BrightSite™ Optical Agents
- High signal-to-noise ratio.
- Low NIR autofluorescence from tissues, cells, biological materials, and drug compounds.
- IRDye 680RD and 800CW absorption/emission near 700 and 800 nm, respectively, matche NIR absorption minima for bodily fluids and tissues, resulting in excellent tissue penetration.
Molar extinction coefficient characteristics of water, hemoglobin and oxygenated hemoglobin (400-1000 nm). IRDye® infrared dyes have ideal excitation/emission wavelengths for in vivo imaging.
IRDye® 680RD EGF Optical Probe is composed of recombinant a EGF polypeptide containing 54 amino acid residues (molecular weight=6.2 kDa) conjugated to the LI-COR IRDye 680RD NIR fluorophore. IRDye® 680RD EGF has been optimized for research pertaining to tumor biology
Properties (in 1X PBS)
- Absorption maximum: 674 nm
- Emission maximum: 698 nm
Brochures & Data Sheets
Webinars
Systematic Evaluation of Targeted IRDye® Labeled Optical Imaging Agents
- Joy Kovar, Sr. ScientistComparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer
- Dr. Eva Sevick-Muraca and Dr. Shi Ke, Baylor College of MedicineCancer Research Webinar Series Part 3: In Vivo Imaging with Near-Infrared Fluorescent Optical Probes
- Dr. Amy GeschwenderCancer Research Webinar Series Part 5: Target Fluorescent Contrast Agents for Image-Guided Cancer Surgery
- Dr. D. Michael Olive
Publications
For related IRDye optical probe-related publications, go to IRDye 800CW EGF Publications.
