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Dyes for Your Near-Infrared Imaging Needs
Figure 1. Imaging of lymph vessels leading to the subiliac lymph nodes with Pearl® Impulse after intradermal administration of IRDye 680RD HA.
IRDye® 680RD HA (Hyaluronan) Optical Probe
IRDye 680RD HA Optical Probe is a near-infrared fluorescent labeled BrightSite™ imaging agent that may be used to target the lymphatic system and those tumors overexpressing CD44 receptors.
Hyaluronan (Hyaluronic acid; HA) is an extracellular matrix glycosaminoglycan formed from disaccharide units containing N-acetylglucosamine and glucuronic acid. Hyaluronan interacts with several cell surface receptors, namely CD44, RHAMM (receptor for Hyaluronan mediated motility; CD168), LYVE-1 (lymphatic vessel endothelial Hyaluronan receptor-1), HARE (Hyaluronan receptor for endocytosis), layilin, and Toll-4. It binds to proteoglycans in cartilage and other tissues and fills an important structural role in the organization of the extracellular matrix. The agent is catabolized by receptor-mediated endocytosis and lysosomal degradation after transport via the lymph to lymph nodes.
Figure 2. Intravenous administration (4 nmol) of IRDye 680RD HA localizes in the lymph nodes systemically as well as liver, kidney and salivary glands. White arrows point to subiliac and accessory axillary lymph nodes 24 h post injection
Advantages of BrightSite™ Optical Agents
- High signal-to-noise ratio.
- Low NIR autofluorescence from tissues, cells, biological materials, and drug compounds.
- IRDye 680RD and 800CW absorption/emission near 700 and 800 nm, respectively, matche NIR absorption minima for bodily fluids and tissues, resulting in excellent tissue penetration.
Molar extinction coefficient characteristics of water, hemoglobin and oxygenated hemoglobin (400-1000 nm). IRDye® infrared dyes have ideal excitation/emission wavelengths for in vivo imaging.
Properties (in 1X PBS)
- Absorption maximum: 671 nm
- Emission maximum: 695 nm
Brochures & Data Sheets
Webinars
Systematic Evaluation of Targeted IRDye® Labeled Optical Imaging Agents
- Joy Kovar, Sr. ScientistCancer Research Webinar Series Part 5: Target Fluorescent Contrast Agents for Image-Guided Cancer Surgery
- Dr. D. Michael OliveCancer Research Webinar Series Part 3: In Vivo Imaging with Near-Infrared Fluorescent Optical Probes
- Dr. Amy GeschwenderComparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer
- Dr. Eva Sevick-Muraca and Dr. Shi Ke, Baylor College of Medicine
Publications
For related IRDye optical probe-related publications, go to IRDye 800CW HA Publications.
