IRDye^® 800CW 2-DG Optical Probe

Figure 1. Two examples in which IRDye 800CW 2-DG Optical Probe (10 nmol) was used to detect subcutaneous A431 tumors. Probe signal is displayed in pseudo color and is merged with a grey scale white light image of the mouse.

Cancer cells are often characterized by a high metabolic rate exemplified by an elevated rate of glycolysis. This observation forms the basis for positron emission tomography (PET) using glucose analogues such as ¹⁸F-2-deoxy-D-glucose (¹⁸FDG) to visualize primary tumors and their metastasis. Radiolabeled 2-deoxy-D-glucose (2-DG) is generally impractical for use in small animal studies of cancer biology. Fluorophore-labeled variants of 2-deoxy-D-glucose have been used with varying degrees of success (O’Neil et al., Lloyd et al., Cheng et al.).

IRDye^® 800CW 2-DG is a fluorescent optical imaging agent that has been shown to be reactive with implanted tumors derived from a number of cell lines including A431, SW620, 3T3-L1, and PC3LMN4. An example is shown in Figure 1. The optical agent exhibits the expected dose response with increasing concentrations of the agent in both cell based and animal experiments. Uptake of the agent can be effectively blocked with either unlabeled 2-DG or glucose, confirming its specificity. Confocal microscopy demonstrated conclusively that IRDye^® 800CW 2-DG is taken up by the cell and localized in the cytoplasm. While the exact mechanism of IRDye^® 800CW 2-DG uptake is not known, there is strong evidence for the involvement of the GLUT transporter system.

Ordering Information:

Advantages of BrightSite™ Optical Agents

• High signal-to-noise ratio.

• Low NIR autofluorescence from tissues, cells, biological materials, and drug compounds.

• IRDye 800CW absorption/emission near 800 nm matches NIR absorption minima for bodily fluids and tissues, resulting in excellent tissue penetration.

Molar extinction coefficent characterisitcs for water, hemoglobin and oxygenated hemoglobin (400-1000 nm). IRDye 800CW has ideal excitation/emission wavelengths for in vivo imaging.

IRDye^® 2-DG has been used for numerous applications, including research pertaining to tumor biology, its metastasis, diabetes and arthritis.

Brochures & Data Sheets

• BrightSite™ Small Animal Imaging Agents Brochure

• BrightSite™ Small Animal Imaging Agents Data Sheet

• Pearl^® Impulse Small Animal Imaging Brochure

Posters

• Ciallella, J. et al
  Near-Infrared Optical Imaging in Mouse Models of Parkinson's Disease, Alzheimer's Disease, and Contact Dermatitis

• Kovar, J.L., et al.
  GLUT Family Transporter Involvement in Cellular Update of IRDye^® 800CW 2-Deoxyglucose

Webinars

• Cancer Research Webinar Series Part 3: In Vivo Imaging with Near-Infrared Fluorescent Optical Probes
  - Dr. Amy Geschwender

• Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer
  - Dr. Eva Sevick-Muraca and Dr. Shi Ke, Baylor College of Medicine

• More Small Animal in vivo Imaging Webinars

Publishing with LI-COR data?

Lowik, C. et al
Optical Image-Guided Surgery – Where do we stand?
Molecular imaging and Biology 2010. Doi: 10.1007/s11307-010-0373-2

Zhao, D et al
Dynamic Near-Infrared Optical Imaging of 2-Deoxyglucose Uptake by Intracranial Glioma of Athymic Mice
PLoS ONE 4:e8051 (2009)

Kovar, J et al
Characterization and Performance of a Near-Infrared 2-Deoxyglucose Optical Imaging Agent for Mouse Cancer Models
Anal Biochem. 384 (2009) 47-54.