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Dyes for Your Near-Infrared Imaging Needs
IRDye® 800CW EGF Optical Probe
Why Target EGFR
IRDye® 800CW EGF Optical Probe is a near-infrared (NIR) labeled recombinant human epidermal growth factor (EGF). Epidermal growth factor receptor (EGFR) is one of a family of receptor tyrosine kinases found on the surface of epithelial cells, to which EGF binds. Many types of cancer cells have abnormally high EGFR levels on the cell surface (Table 1). In vivo assays of EGF binding have proven effective in monitoring specific solid tumor models that exhibit over-expression of EGFR2-6. EGF conjugated to LI-COR's IRDye 800CW NIR fluorophore provides a versatile probe that can be used for in vitro and in vivo assays, as well as whole organ and tissue section analysis.
Ordering Information
Please see our new Online Catalog for the latest ordering information.For research purposes only. Not for diagnostic or therapeutic use. Not for use in humans. LI-COR IRDye 800 and other LI-COR reagent products are covered by U.S. patents, foreign equivalents, and patents pending.
Shipping information:U.S.A.: Same-day shipping (Federal Express overnight) available on orders received before 12:00 noon CST.
Canada: Contact Mandel Scientific.
Germany & UK: Contact LI-COR GmbH.
International: Contact a LI-COR distributor in your area All shipments subject to availability.
IRDye is a registered trademark of LI-COR Inc.
Advantages of BrightSite™ Optical Agents
- High signal-to-noise ratio.
- Low NIR autofluorescence from tissues, cells, biological materials, and drug compounds.
- IRDye 800CW absorption/emission near 800 nm matches NIR absorption minima for bodily fluids and tissues, resulting in excellent tissue penetration.
Molar extinction coefficient characteristics of water, hemoglobin and oxygenated hemoglobin (400-1000 nm). IRDye® infrared dyes have ideal excitation/emission wavelengths for in vivo imaging.
IRDye® 800CW EGF Optical Probe is composed of recombinant a EGF polypeptide containing 54 amino acid residues (molecular weight=6.2 kDa) conjugated to the LI-COR IRDye 800CW NIR fluorophore. IRDye® 800CW EGF has been optimized for research pertaining to tumor biology
Probe Specificity
In-Cell Western™ assays on the LI-COR Odyssey® and Aerius® Infrared Imaging Systems are used to determine binding specificity of the probe in vitro for various cell lines (Figure 1). IRDye 800CW EGF has been characterized in A431 (squamous epithelial carcinoma, Figure 2), and PC3M-LN4 and 22Rv1 (human prostate adenocarcinoma) cell lines that over-express EGFR.
Figure 1 - Two different cell lines known to over-express the EGFR were characterized by evaluating:
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1. binding of IRDye 800CW EGF;
2. IRDye 800CW only;
3. competition assay using unlabeled EGF to compete for EGFR sites
Figure 2 - Two Athymic Nu/Nu tumor-bearing mice were injected with either 0.9% saline (left) or 1 nmol IRDye 800CW EGF (right) 72 hours prior to imaging with the LI-COR Odyssey Infrared Imaging System
Brochures & Data Sheets
Application Note
Posters
Kovar, J. L., et al.
EGF-IRDye 800CW: in vitro and in vivo characterization as a biomarker for optical fluorescent imaging of tumor growth kineticsKovar, J. L., et al.
Monitoring progression of prostate tumors in mice by receptor-targeted near infrared optical imaging
Webinars
Systematic Evaluation of Targeted IRDye® Labeled Optical Imaging Agents
- Joy Kovar, Sr. ScientistComparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer
- Dr. Eva Sevick-Muraca and Dr. Shi Ke, Baylor College of MedicineCancer Research Webinar Series Part 3: In Vivo Imaging with Near-Infrared Fluorescent Optical Probes
- Dr. Amy GeschwenderCancer Research Webinar Series Part 5: Target Fluorescent Contrast Agents for Image-Guided Cancer Surgery
- Dr. D. Michael Olive
Lowik, C. et al
Optical Image-Guided Surgery – Where do we stand?
Molecular imaging and Biology 2010. Doi: 10.1007/s11307-010-0373-2Gibbs-Strauss, SL et al.
Detecting Epidermal Growth Factor Receptor Tumor Activity In Vivo During Cetuximab Therapy of Murine Gliomas
Acad Radiol (17): 7-17 (2010)Bharadwaj, AG et al.
Spontaneous metastasis of prostate cancer is promoted by excess hyaluronan synthesis and processing
Am J Pathol. 174(3):1027-36. (2009)Offterdinger, M et al.
Prolonged EGFR Signaling by ERBB2-Mediated Sequestration at the Plasma Membrane
Traffic. 9(1):147-55 (2008)Adams, KE et al.
Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer
J Biomed Opt. 12(2):024017 (2007)Kovar, J et al.
Purification method directly influences effectiveness of an epidermal growth factor-coupled targeting agent for noninvasive tumor detection in mice
Anal Biochem. 361(1):47-54 (2007)Kovar, J et al.
A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models
Anal Biochem. 367():1-12 (2007)Kovar, J et al.
Hyaluronidase expression induces prostate tumor metastasis in an orthotopic mouse model
Am J Pathol. 169(4):1415-26 (2006)Kovar, J et al.
Hyaluronidase Expression Induces Prostate Tumor Metastasis in an Orthotopic Mouse Model
American Journal of Pathology 2006;169:1415-1426
