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Dyes for Your Near-Infrared Imaging Needs
Figure 1. Imaging of lymph vessels leading to the subiliac lymph nodes with Pearl® Impulse approximately 1h after intradermal administration of IRDye 800CW HA. High signal intensity in pseudo-colored image is represented in red whereas low signal intensity is represented by blue.
IRDye® 800CW HA (Hyaluronan) Optical Probe
IRDye 800CW HA may be used to target the lymphatic system and those tumors over-expressing CD44 receptors.
Hyaluronan (Hyaluronic acid; HA) is an extracellular matrix glycosaminoglycan formed from disaccharide units containing N-acetylglucosamine and glucuronic acid. Hyaluronan interacts with several cell surface receptors, namely CD44, RHAMM (receptor for Hyaluronan mediated motility; CD168), LYVE-1 (lymphatic vessel endothelial Hyaluronan receptor-1), HARE (Hyaluronan receptor for endocytosis), layilin, and Toll-4. It binds to proteoglycans in cartilage and other tissues and fills an important structural role in the organization of the extra-cellular matrix. The agent is catabolized by receptor-mediated endocytosis and lysosomal degradation after transport via the lymph to lymph nodes.
Figure 2. Intravenous administration of IRDye 800CW HA (2 nmol) localizes in the lymph nodes systemically (A) as well as liver, kidney and salivary glands (B). Image captured 14 d post injection.
Figure 3. Cell-surface receptor binding of IRDye 800CW HA and Sytox green nuclear stain. Panel A shows 800nm image representing the probe (green), Panel B includes the nuclear stain, Sytox green (blue, 511 nm) and Panel C adds the DIC channel shown in red. Images were captured using Olympus IX81 Inverted Microscope equipped with a halogen bulb and NIR filters (EX:710/75, DC:760LP, EM:810/90; Chroma Technology Corp., Rockingham, VT).
IRDye 800CW HA Specifications
IRDye® 800CW HA: 20 nmol per tube (~10 doses)
| IRDye 800CW (1X PBS) | |
|---|---|
| Absorption Maximum (nm) Peak A | 701 |
| Absorption Maximum (nm) Peak B | 775 |
| Emission Maximum (nm) | 789 |
Note: The absorbance spectra for IRDye 800CW HA bares two distinct peaks. The first absorbance peak (701 nm) is due to interaction among the labeled dye on the polymer chains and the second peak (775 nm) is the product peak.
Precautions
IRDye 800CW HA clearance is slow, with significant retention in liver and salivary glands.
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Brochures & Data Sheets
Webinars
Systematic Evaluation of Targeted IRDye® Labeled Optical Imaging Agents
- Joy Kovar, Sr. ScientistCancer Research Webinar Series Part 5: Target Fluorescent Contrast Agents for Image-Guided Cancer Surgery
- Dr. D. Michael OliveComparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer
- Dr. Eva Sevick-Muraca and Dr. Shi Ke, Baylor College of MedicineCancer Research Webinar Series Part 3: In Vivo Imaging with Near-Infrared Fluorescent Optical Probes
- Dr. Amy Geschwender
Kuo, J.W.
Practical Aspects of Hyaluronan Based Medical Products. CRC Press, Taylor & Francis Group; Boca Raton, FL. (2006)
Stern, R. (Ed)
Hyaluronan in Cancer Biology. Academic Press, Elsevier, San Diego, CA. (2009)
