IRDye® 800RS Casein Protease Substrate
IRDye® 800RS Casein Protease Substrate is a general substrate used to detect protease activity. Casein a protein found in milk, is over-labeled with IRDye 800RS producing a quenched substrate for use in protease assays. This near-infrared protease substrate has significant advantages compared to protease assays using visible fluorescence.
Advantages:
Simple - Incubate sample or known protease withlabeled substrate, measure fluorescence, quantify protease concentration from standard curve.
Enhanced assay sensitivity due to low infrared background fluorescence
Requires a fraction of the substrate compared to visible fluorescence kits
Improved detection limits of protease activity
Up to 20-fold fluorescence intensity enhancement upon digestion
Can be used for protease inhibitor screening
Versatile - No stop solution to add and fluorescence is detected at the end of incubation. System allows for monitoring reactions over time.
Assay Description
When the IRDye 800RS Casein Substrate is over-labeled with IRDye 800RS, the IRDye 800RS fluorophores are quenched due to intramolecular interactions of dye molecules in close proximity to each other.
Figure 1. Schematic of IRDye 800RS Casein
Protease Substrate. Fluorescence increases upon protease
substrate digestion.
Figure 2. A, B, and C are three replicates. Column 1:
buffer only; column 2: trypsin only; and column 3: the
substrate only, IRDye 800RS Casein. A two-fold dilution
series of trypsin from 5 µg/mL to 19.7 ng/mL is shown
in columns 4-12 respectively, at a substrate concentration
of 20 nM.
Ordering Information:
| Part # | Description | Unit/Size | Downloads |
| 926-08587 | IRDye Casein Protease Substrate
Kit contains: IRDye 800RS Casein, 4 tubes lyophilized, store at -20°C. Each tube contains 6.3 nmol of fluorescent dye (~25 µg protein) in 20 mM potassium phosphate buffer, ph 8.5 (~700 nmol total potassium phosphate). Sodium Azide, 0.5% (w/v) solution, store at 4°C Each package contains one tube of 0.5% sodium azide solution (100 µl).
Shipping: Ice Packs Storage: -20/4°C depending on component | 1000 Reactions | datasheet
description
msds
pack insert
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| 926-09116 | IRDye Casein Protease Substrate (Trial Size)
Kit contains: IRDye 800RS Casein, 1 tube lyophilized, store at -20°C
Each tube contains 6.3 nmol of fluorescent dye (~25 µg protein) in 20 mM potassium phosphate buffer, ph 8.5 (~700 nmol total potassium phosphate). < Sodium Azide, 0.5% (w/v) solution, store at 4°C
Each package contains one tube of 0.5% sodium azide solution (100 µl).
Shipping: Ice Packs Storage: -20/4°C depending on component | 250 Reactions | datasheet
description
msds
pack insert
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