Fluorescent Secondary Antibodies for Western blots, Protein Arrays, Immunofluorescent Assays.

LI-COR Biosciences offers a range of secondary antibodies for Western blotting, In-Cell Western™ Assay, immunohistochemistry, and many other applications. IRDye secondary antibodies are highly cross-adsorbed, making them suitable for two-color detection. They have been optimized for use with the Odyssey family of imaging systems and can be used on instruments with similar excitation and emission filters.

IRDye 800CW Secondary Antibodies

IRDye 800CW secondary antibodies are the antibodies of choice for a wide variety of applications in the 800 channel. IRDye 800CW secondary antibodies can be used for 2-color detection when multiplexed with IRDye 680RD or IRDye 680LT secondary antibodies.

Highly Recommended for:

Western Blot
In-Cell Western Assay
On-Cell Western Assay
Protein Array
Immunohistochemistry
Microscopy
2D Gel Detection
Tissue Section Imaging
Small Animal Imaging
Virus Titration Assay
FRET-Based Assays

NEW! IRDye 680RD Secondary Antibodies

IRDye 680RD secondary antibodies are the antibodies of choice for In-Cell Western Assay and Western blot applications in the 700 nm channel. These antibodies can be used for 2-color detection when multiplexed with IRDye 800CW secondary antibodies. These antibodies are our most universal use 700 nm channel antibodies.

Highly Recommended for:

Western Blot
In-Cell Western Assay
On-Cell Western Assay
Protein Array
Immunohistochemistry
Microscopy
2D Gel Detection
Tissue Section Imaging
Small Animal Imaging

IRDye 680LT Secondary
Antibodies

IRDye 680LT secondary antibodies have been proven the brightest signal for Western blot detection in the 700 channel and are comparable to Alexa Fluor 680 secondary antibodies. They are an excellent choice for low abundance targets and can be used for 2-color detection when multiplexed with IRDye 800CW secondary antibodies.

Recommended for:

Western Blot
Protein Array
Immunohistochemistry
Microscopy
2D Gel Detection
Tissue Section Imaging

Not Recommended for:

In-Cell Western Assay
On-Cell Western Assay
Small Animal Imaging

Part No.	Description
926-32210	IRDye 800CW Goat anti-Mouse IgG (H + L), 0.5 mg
926-32211	IRDye 800CW Goat anti-Rabbit IgG (H + L), 0.5 mg
926-32212	IRDye 800CW Donkey anti-Mouse IgG (H + L), 0.5 mg
926-32213	IRDye 800CW Donkey anti-Rabbit IgG (H + L), 0.5 mg
926-32214	IRDye 800CW Donkey anti-Goat IgG (H + L), 0.5 mg
926-32218	IRDye 800CW Donkey anti-Chicken IgG (H + L), 0.5 mg
926-32219	IRDye 800CW Goat anti-Rat IgG (H + L), 0.5 mg
926-32411	IRDye 800CW Donkey anti-Guinea Pig IgG (H + L), 0.5 mg
926-32232	IRDye 800CW Goat anti-Human IgG (H + L), 0.5 mg
926-32350	IRDye 800CW Goat anti-Mouse IgG₁ Specific, 0.5 mg
926-32351	IRDye 800CW Goat anti-Mouse IgG_2a Specific, 0.5 mg
926-32352	IRDye 800CW Goat anti-Mouse IgG_2b Specific, 0.5 mg
926-68070	IRDye 680RD Goat anti-Mouse IgG (H + L), 0.5 mg
926-68071	IRDye 680RD Goat anti-Rabbit IgG (H + L), 0.5 mg
926-68072	IRDye 680RD Donkey anti-Mouse IgG (H + L), 0.5 mg
926-68073	IRDye 680RD Donkey anti-Rabbit IgG (H + L), 0.5 mg
926-68074	IRDye 680RD Donkey anti-Goat IgG (H + L), 0.5 mg
926-68075	IRDye 680RD Donkey anti-Chicken IgG (H + L), 0.5 mg
926-68076	IRDye 680RD Goat anti-Rat IgG (H + L), 0.5 mg
926-68077	IRDye 680RD Donkey anti-Guinea Pig IgG (H + L), 0.5 mg
926-68078	IRDye 680RD Goat anti-Human IgG (H + L), 0.5 mg
926-68020	IRDye 680LT Goat anti-Mouse IgG (H + L), 0.5 mg
926-68021	IRDye 680LT Goat anti-Rabbit IgG (H + L), 0.5 mg
926-68022	IRDye 680LT Donkey anti-Mouse IgG (H + L), 0.5 mg
926-68023	IRDye 680LT Donkey anti-Rabbit IgG (H + L), 0.5 mg
926-68024	IRDye 680LT Donkey anti-Goat IgG (H + L), 0.5 mg
926-68028	IRDye 680LT Donkey anti-Chicken IgG (H + L), 0.5 mg
926-68029	IRDye 680LT Goat anti-Rat IgG (H + L), 0.5 mg
926-68030	IRDye 680LT Donkey anti-Guinea Pig IgG (H + L), 0.5 mg
926-68032	IRDye 680LT Goat anti-Human IgG (H + L), 0.5 mg
926-68050	IRDye 680LT Goat anti-Mouse IgG₁ Specific, 0.5 mg
926-68051	IRDye 680LT Goat anti-Mouse IgG_2a Specific, 0.5 mg
926-68052	IRDye 680LT Goat anti-Mouse IgG_2b Specific, 0.5 mg