NHS Esters

NHS Esters

Near-Infrared Fluorescence Labeling Reagents

LI-COR IRDye^® infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence. These unique dyes are currently used in a variety of NIR imaging applications including DNA sequencing, microsatellites, protein research, and small animal imaging. Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes.

IRDye^® 800CW

IRDye 800CW is the dye of choice for protein/antibody labeling applications and for nucleic acid applications requiring high labeling density. The NHS ester reactive group provides the functionality for labeling primary and secondary amino groups. This dye has the highest water solubility and salt tolerance of the IRDye 800 series.

If you are interested in commercial development rights using IRDye infrared dyes, please contact Business Development.

Dye Properties: Overview

Data are for methanol only and are solvent-dependent. View dye properties for more information.

	Extinction Coefficient	Max. Abs. (nm)	Max. Emis. (nm)	Properties
IRDye 800CW	300,000	778	794	View Properties
IRDye 800RS	300,000	770	786	View Properties
IRDye 700DX	210,000	680	687	View Properties

Ordering Information:

Please see our new Online Catalog for the latest ordering information.

LI-COR Infrared Dye Successfully Completes Toxicity Studies

September 10, 2007: LINCOLN, Neb. – LI-COR® Biosciences announces successful completion of animal toxicity studies of its IRDye® 800CW infrared dye carboxylic acid using a protocol reviewed by the Food and Drug Administration. The completion of the toxicity studies is a key milestone in the development of the IRDye 800CW dye for potential clinical imaging use.
 
LI-COR IRDye infrared fluorescent dyes are used for protein and cellular assays, microscopy, and in vivo molecular imaging in animals for research purposes.  IRDye 800CW is cited in a wide variety of published non-clinical research for labeling nucleic acids, antibodies, proteins, and peptides where there is a need for high signal, low background imaging. With an 800 nm emission wavelength, the IRDye 800CW dye is spectrally ideal for animal imaging research.

For more than 16 years scientists have used LI-COR infrared dyes for research including drug discovery, cell signaling analysis, animal imaging, and the Human Genome Project. LI-COR develops instrumentation, analysis software, and infrared reagents offering researchers a complete solution for protein analysis, genomics, and animal imaging.

“The unique characteristics of the IRDye 800CW infrared dye make it an excellent choice for molecular and small animal research,” says Jeff Harford, LI-COR product manager. “Completing the animal toxicity studies is the logical next step in the development of the IRDye 800CW infrared dye for use in the clinical setting and is critical to our preparation for future clinical investigations.”   

While the IRDye 800CW dye has successfully completed toxicity studies, it has not been studied for diagnostic or therapeutic use in humans, and has not been approved by the Food and Drug Administration for this use.  LI-COR is exploring options for additional studies as next steps in the Food and Drug Administration approval process leading toward clinical use.

LI-COR Reports on Toxicity Study of its IRDye 800CW Carboxylate at Global Imaging Summit in Berlin

October 22, 2009:Lincoln, NE/Berlin, Germany: LI-COR Biosciences presented preclinical data on its investigational IRDye® 800CW carboxylate, which is being developed for potential use in clinical applications. To prepare for clinical use as a near-infrared (NIR) fluorophore, a safety study was conducted on IRDye 800CW carboxylate with Sprague Dawley rats. Michael Olive, LI-COR vice president of science and technology, presented the findings of the study at the Global Imaging Summit in Berlin.

“With the current environment of NIR fluorescence instrument development, IRDye 800CW could impact molecular imaging by providing a functionalized NIR dye enabling a variety of new targeting contrast agents,” reports Olive. “Based on hematologic and clinical chemistry evaluations, and histopathologic evaluation of tissues from animals in this study, single administration of IRDye 800CW carboxylate intravenously at dose levels of 1, 5 and 20 mg/kg or 20 mg/kg intradermally followed by up to 14 days of observation produced no pathological evidence of toxicity.”

LI-COR is preparing to submit a Drug Master File on IRDye 800CW to the U.S. Food and Drug Administration. LI-COR anticipates having dye made in compliance with cGMP and available for clinical studies in early 2010.

Optical imaging using near-infrared fluorophores has the potential for use in endoscopy, intraoperative surgical guidance, and imaging the lymphovascular system.

LI-COR Biosciences is a leading provider of near-infrared reagent and detection systems. LI-COR provides complete research solutions for proteomic, genomic and small animal imaging research areas. LI-COR IRDye infrared dyes, optical agents and reagents are well established in the research and pre-clinical markets especially for use in quantitative western blot analysis and in vivo imaging in small animals.

About LI-COR Biosciences: LI-COR Biosciences is a leader in the design and manufacture of instrument systems for biotechnology, plant biology and environmental research. LI-COR is a pioneer in the development of infrared fluorescence labeling and detection systems for such areas as drug discovery and DNA sequencing for genomic research.  The Odyssey® and Aerius® systems offer unique infrared imaging solutions for protein analysis of Westerns, micro plate-based assays, and in vivo imaging.

Founded in 1971, the privately held company is based in Lincoln, Nebraska, with subsidiaries in Germany and the United Kingdom. LI-COR systems are used in more than 100 countries and are supported by a global network of distributors. Further information can be obtained at www.licor.com.