NHS Esters

NHS Esters

Near-Infrared Fluorescence Labeling Reagents

IRDye^® infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence. These unique dyes are used in a variety of NIR imaging applications including DNA sequencing, microsatellites, protein research, Western blotting, plate-based assays, and small animal imaging. Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amino groups.

If you are interested in commercial development rights using IRDye infrared dyes, please contact Business Development.

Dye Properties: Overview

Table 1. Data are for methanol only and are solvent-dependent. View dye properties for more information.

	Extinction Coefficient	Max. Abs. (nm)	Max. Emis. (nm)	Properties
IRDye 800CW	300,000	778	794	View Properties
IRDye 680RD	170,000	680	694	View Properties
IRDye 680LT	250,000	680	694	View Properties
IRDye 750	330,000	766	776	View Properties
IRDye 700DX	210,000	680	687	View Properties
IRDye 800RS	300,000	770	786	View Properties
IRDye 650	240,000	651	668	View Properties

Ordering Information:

Please see our new Online Catalog for the latest ordering information.

LI-COR Translational Research

LI-COR Translational Research is working to facilitate the use of IRDye^® 800CW-labeled imaging agents in clinical studies for fluorescence-assisted cancer surgery. In preclinical studies, IRDye 800CW has been conjugated to a variety of targeted agents by a number of leading molecular imaging laboratories with the intent to translate these to clinical use.

Published Study: Single-Dose Intravenous Toxicity Study of IRDye 800CW in Sprague-Dawley Rats, Marshall, M.V., et al. (2010)

LI-COR Announces IRDye^® 800CW Near-Infrared Dye Manufactured for Use in Drug Development

September 8, 2010, Lincoln, NE: LI-COR^® Biosciences announces it has completed manufacturing of IRDye^® 800CW near-infrared dye under a GMP process. This is considered a key step toward translating near-infrared contrast agents into clinical use... [Read More]

LI-COR Biosciences Establishes Translational Research Group

June 9, 2010, Lincoln, NE: LI-COR^® Biosciences announces the establishment of a new Translational Research group to facilitate the use of its IRDye 800CW labeled imaging agents in clinical studies for detection of disease, its progression, and for monitoring treatment and drug efficacy. IRDye 800CW has been conjugated to a variety of preclinical targeting agents by leading molecular imaging laboratories developing translatable agents. Unlike the clinically approved form of indocyanine green (ICG, Cardiogreen^®), which cannot be attached to biomolecules, IRDye 800CW reactive dyes are easily conjugated to targeting agents... [Read More]

Study Published on Toxicity of IRDye^® 800CW Carboxylate in Sprague Dawley Rats

April 21, 2010, Lincoln, NE: In preparation for potential clinical use, a paper reporting a safety and toxicity study of the near infrared dye IRDye^® 800CW was reported April 8th in the online section of Molecular Imaging and Biology. The manuscript entitled, "Single-Dose Intravenous Toxicity Study of IRDye 800CW in Sprague-Dawley Rats" reported no pathological evidence of toxicity based on the hematologic, clinical chemistry, and histopathologic analyses performed in the study... [Read More]

LI-COR Infrared Dye Successfully Completes Toxicity Studies

September 10, 2007, Lincoln, NE: LI-COR^® Biosciences announces successful completion of animal toxicity studies of its IRDye^® 800CW infrared dye carboxylic acid using a protocol reviewed by the Food and Drug Administration. The completion of the toxicity studies is a key milestone in the development of the IRDye 800CW dye for potential clinical imaging use... [Read More]