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LI-COR REAGENTS
PSVue® 794 Reagent Kit
Reagents for LI-COR Imaging Systems
PSVue® 794 Reagent Kit
PSVue® 794 is a near-infrared fluorescent probe for detection of apoptotic and necrotic cells, bacteria and other anionic membranes. The PSVue 794 (formerly PSS 794) reagent kit contains components to provide a 1 mM solution of PSVue794 in aqueous solution. The compound exhibits fluorescence excitation maximum at 794nm and emission maximum at 810 nm and through its zinc(II)-dipicolylamine (Zn-DPA) moiety, it has been found to bind strongly to negatively charged bacterial cell walls (e.g. S. aureus, E. coli) and necrotic regions present in various tumors (e.g. mammary, prostate, glioma) in vitro and in vivo. In particular, it has also been found to bind to the phosphatidylserine (PS) residues exposed on the cell surface of apoptotic cells, making it a more cost-effective alternative to fluorescently labeled Annexin V in various cell death assays. The labeling vehicle provided with the kit (Diluent X) is designed to maximize dye solubility and is suitable for in vitro and in vivo use.
The kit contains:
1 vial containing pre-weighed amount of apo-PSS 794 solid dye (at least 1 mg)
1 vial of Diluent X (1 mL)
1 vial of 4.2 mM zinc nitrate solution in Diluent X (1 mL)
Figure 1. MPTP was used to induce cell death in mouse brains as a model for Parkinson's Disease. C57BI/6 mice were treated with MPTP to selectively destroy dopaminergic neurons. Mice were then injected with PSVue® dye or control dye and imaged on the Pearl® Imager 68 hrs post injection. A. control (i.e. non-targeting) dye; B. and C. PSVue® dye; D. excised brains from the three animals
Near-Infrared Optical Imaging in Mouse Models of Parkinson's Disease, Alzheimer's Disease, and Contact Dermatitis
PSVue is a registered trademark of MTTI, used under license. PSVue is sold under license from MTTI. US Patent # 7,179,616 and others pending.
PSVue® 794 is a near-infrared fluorescent dye for detection of apoptotic cells, bacteria, and other anionic membranes.
Figure 1. Structure of apo-PSS 794, precursor to PSVue 794.
Figure 2. PSVue 794 Absorption and Fluorescence Emission Spectra (5 µM solution; abs. max = 794 nm; fl. em max = 810 nm.
