Overview
The Quick Western Kit — IRDye® 680RD provides a universal detection reagent that can be combined with the primary antibody incubation step, eliminating the need for a secondary antibody incubation step.
This reduces the overall time to complete a Western blot and provides the advantages of near infrared fluorescence detection.
Saves Time (View Workflow)
- Does not require a separate primary antibody labeling step, saving time and antibody
- Faster method of detection compared to the traditional 2-step method, which can take up to 4 hours
- Reduces the total Western blot procedure by at least 1.5 hours
Convenient
- Eliminates the use of labeled secondary antibodies and associated washes
- Eliminates the need to purchase multiple secondary antibodies
- Quick, quantitative solution
Universal Detection
- Works with a variety of primary antibodies (see list below)
- Has been shown to recognize primary antibodies to recombinant tagged proteins (i.e. 6X His, Myc, FLAG, etc.)
IRDye 680RD Detection Reagent is known to have high specificity for IgG from:
- Human
- Mouse
- Rabbit
- Guinea Pig
- Goat
- Sheep
- Pig
- Cow
- Cat
- Dog
- Donkey
-
The Detection Reagent has been specifically tested and qualified for Western blot applications. If additional specificity and/or affinity are required, please use IRDye secondary antibodies for detection.
Product
- Quick Western Kit — IRDye® 680RD [Part # 926-68100]
[Above] Two-fold dilutions of NIH/3T3 lysate (20 µg - 2 ng) were resolved on 10% Bis-Tris reducing gel and transferred to Odyssey® Nitrocellulose. The Quick Western Kit protocol was followed and the membrane was probed with anti-p38 Rabbit Antibody (CST; 1:1000) and IRDye 680RD Detection Reagent (1:1000) for 1 hour. The image was collected on the Odyssey CLx.
[Above] Two-fold dilutions of crude lysate containing an overexpressed 6X-His tagged DNA polymerase was loaded in Lanes 3-7. A Histag molecular weight marker (Invitrogen) was loaded in Lanes 1 & 9. The nitrocellulose membrane was blocked with Odyssey® Blocking Buffer and probed with anti-His Tag Rabbit Polyclonal Antibody (GenScript; 1:500) and IRDye 680RD Detection Reagent (1:1000) for 1 hour. The image was collected on the Odyssey CLx.
