Western Blotting Kits
Reagents and Membranes for Quantitative Westerns
Infrared fluorescence detection has changed Western blotting (immunoblotting) by providing unparalleled
quantification accuracy and simultaneous two-color multiplexed detection. Simultaneous two-color detection allows detection of both total protein as well as the amount of protein phosphorylation without stripping and reprobing.
LI-COR’s Western blotting kits provide a convenient way to purchase reagents and membranes that are optimized for infrared fluorescence detection on the Odyssey and Aerius infrared imaging systems. The Western blotting kits provide enough secondary antibodies, blocking buffer, and membranes for 10 Western blots. Kits are available with either PVDF or nitrocellulose membranes. Secondary antibodies in each kit are cross-absorbed for multiplex detection and provide high specificity.
Kits with PVDF Membranes
IRDye Western Kit I (part #926-31050)
- IRDye 800CW Goat Anti-Mouse Secondary Antibody (25 µl, 1mg/ml)
- IRDye 680 Goat Anti-Rabbit Secondary Antibody (25 µl, 1mg/ml)
- 500 ml Odyssey Blocking Buffer
- 10 Millipore Immobilon™-FL PVDF Membranes (0.45 µm, 10 x 10 cm)
IRDye Western Kit II (part #926-31052)
- IRDye 800CW Goat Anti-Rabbit Secondary Antibody (25 µl, 1mg/ml)
- IRDye 680 Goat Anti-Mouse Secondary Antibody (25 µl, 1mg/ml)
- 500 ml Odyssey Blocking Buffer
- 10 Millipore Immobilon-FL PVDF Membranes (0.45 µm, 10 x 10 cm)
Kits with Nitrocellulose Membranes
IRDye Western Kit III (part #926-31058)
- IRDye 800CW Goat Anti-Mouse Secondary Antibody (25 µl, 1mg/ml)
- IRDye 680 Goat Anti-Rabbit Secondary Antibody (25 µl, 1mg/ml)
- 500 ml Odyssey Blocking Buffer
- 10 Odyssey Nitrocellulose Membranes (0.22 µm, 7 x 8.5 cm)
IRDye Western Kit IV (part #926-31060)
- IRDye 800CW Goat Anti-Rabbit Secondary Antibody (25 µl, 1mg/ml)
- IRDye 680 Goat Anti-Mouse Secondary Antibody (25 µl, 1mg/ml)
- 500 ml Odyssey Blocking Buffer
- 10 Odyssey Nitrocellulose Membranes (0.22 µm, 7 x 8.5 cm)
Ordering Information
Please see our new Online Catalog for the latest ordering information.
Shipping information:
U.S.A.: Same-day shipping (Federal Express overnight) available on orders received before 12:00 noon CST.
Canada: Contact Mandel Scientific.
Germany & UK: Contact LI-COR GmbH.
International: Contact a LI-COR distributor in your area All shipments subject to availability.
