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LI-COR REAGENTS
Western Blotting Kits
Reagents for LI-COR Imaging Systems
Western Blotting Kits
Reagents and Membranes for Quantitative Westerns
Infrared fluorescence detection has changed Western blotting (immunoblotting) by providing unparalleled
quantification accuracy and simultaneous two-color multiplexed detection. Simultaneous two-color detection allows detection of both total protein as well as the amount of protein phosphorylation without stripping and reprobing.
[ABOVE] In this pseudocolored image, 700 nm fluorescence (IkappaB) is shown in red and 800 nm fluorescence (Tubulin) is shown in green. The two colors were imaged simultaneously in a single scan
Data courtesy of Dr. Catrin Albrecht, IUF, Germany
LI-COR’s Western blotting kits provide a convenient way to purchase reagents and membranes that are optimized for infrared fluorescence detection on the Odyssey infrared imaging systems. The Western blotting kits provide enough secondary antibodies, blocking buffer, and membranes for 10 Western blots. Kits are available with either PVDF or nitrocellulose membranes. Secondary antibodies in each kit are cross-absorbed for multiplex detection and provide high specificity.
Kits with PVDF Membranes
Odyssey Western Blotting Kit I LT (P/N 926-31062)
- IRDye 800CW Goat Anti-Mouse Secondary Antibody (25 µl, 1mg/ml)
- IRDye 680LT Goat Anti-Rabbit Secondary Antibody (25 µl, 1mg/ml)
- 500 ml Odyssey Blocking Buffer
- 10 Millipore Immobilon™-FL PVDF Membranes (0.45 µm, 10 x 10 cm)
Odyssey Western Blotting Kit II LT (P/N 926-31064)
- IRDye 800CW Goat Anti-Rabbit Secondary Antibody (25 µl, 1mg/ml)
- IRDye 680LT Goat Anti-Mouse Secondary Antibody (25 µl, 1mg/ml)
- 500 ml Odyssey Blocking Buffer
- 10 Millipore Immobilon™-FL PVDF Membranes (0.45 µm, 10 x 10 cm)
Odyssey Western Blotting Kit I (P/N 926-31050)
- IRDye 800CW Goat Anti-Mouse Secondary Antibody (25 µl, 1mg/ml)
- IRDye 680LT Goat Anti-Rabbit Secondary Antibody (25 µl, 1mg/ml)
- 500 ml Odyssey Blocking Buffer
- 10 Millipore Immobilon™-FL PVDF Membranes (0.45 µm, 10 x 10 cm)
Odyssey Western Blotting Kit II (P/N 926-31052)
- IRDye 800CW Goat Anti-Rabbit Secondary Antibody (25 µl, 1mg/ml)
- IRDye 680LT Goat Anti-Mouse Secondary Antibody (25 µl, 1mg/ml)
- 500 ml Odyssey Blocking Buffer
- 10 Millipore Immobilon™-FL PVDF Membranes (0.45 µm, 10 x 10 cm)
Kits with Nitrocellulose Membranes
Odyssey Western Blotting Kit III LT (P/N 926-31066)
- IRDye 800CW Goat Anti-Mouse Secondary Antibody (25 µl, 1mg/ml)
- IRDye 680LT Goat Anti-Rabbit Secondary Antibody (25 µl, 1mg/ml)
- 500 ml Odyssey Blocking Buffer
- 10 Odyssey Nitrocellulose Membranes (0.22 µm, 7 x 8.5 cm)
Odyssey Western Blotting Kit IV LT (P/N 926-31068)
- IRDye 800CW Goat Anti-Rabbit Secondary Antibody (25 µl, 1mg/ml)
- IRDye 680LT Goat Anti-Mouse Secondary Antibody (25 µl, 1mg/ml)
- 500 ml Odyssey Blocking Buffer
- 10 Odyssey Nitrocellulose Membranes (0.22 µm, 7 x 8.5 cm)
Ordering Information
Please see our new Online Catalog for the latest ordering information. Shipping information:U.S.A.: Same-day shipping (Federal Express overnight) available on orders received before 12:00 noon CST.
Canada: Contact Mandel Scientific.
Germany & UK: Contact LI-COR GmbH.
International: Contact a LI-COR distributor in your area All shipments subject to availability.
