LI-COR Biosciences - IRDye® Protein Labeling Kits

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IRDye® Protein Labeling Kits label antibodies and other proteins for applications such as Western blots, In-Cell Western™ assays, in vivo imaging, and whole organ or tissue section assays. IRDye labeling kits are cost-effective alternatives to expensive, time-consuming, custom antibody labeling services.

Labeling Reactions

IRDye reactive dyes bear an N-hydroxysuccinimide (NHS) reactive group that couples to free amino groups and forms a stable conjugate. Proteins other than IgG antibodies can be labeled, but protocol adjustment and optimization may be necessary.

IRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Simply dissolve the protein in the provided buffer, combine it with the appropriate amount of water soluble amine-reactive IRDye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.

Ordering Information:

Part #	Description	Unit/Size	Downloads	Order Online
928-38040	IRDye 800CW Protein Labeling Kit - High Molecular Weight Contents: 3x IRDye 800CW reactive dye vials (0.1 mg) 1x 0.5 ml 1M potassium phosphate 1x 25 ml 1X PBS 1x 0.5 ml ultra-pure water 3x desalting spin columns	1 kit	description msds pack insert	$ 350.00
928-38042	IRDye 800CW Protein Labeling Kit - Low Molecular Weight Contents: 3x IRDye 800CW reactive dye vials (0.5 mg) 1x 0.5 ml 1M potassium phosphate 1x 25 ml 1X PBS 1x 0.5 ml ultra-pure water 3x desalting spin columns	1 kit	description msds pack insert	$ 395.00
928-38044	IRDye 800CW Protein Labeling Kit - Microscale Contents: 3x IRDye 800CW reactive dye vials (0.1 mg) 1x 0.5 ml 1M potassium phosphate 1x 25 ml 1X PBS 1x 0.5 ml ultra-pure water 3x desalting spin columns	1 kit	description msds pack insert	$ 295.00
928-38046	IRDye 700DX Protein Labeling Kit - High Molecular Weight Contents: 3x IRDye 700DX reactive dye vials (0.5 mg) 1x 0.5 ml 1M potassium phosphate 1x 25 ml 1X PBS 1x 0.5 ml ultra-pure water 3x desalting spin columns	1 Kit	description msds pack insert	350
928-38048	IRDye 700DX Protein Labeling Kit - Low Molecular Weight Contents: 3x IRDye 700DX reactive dye vials (0.5 mg) 1x 0.5 ml 1M potassium phosphate 1x 25 ml 1X PBS 1x 0.5 ml ultra-pure water 3x desalting spin columns	1 Kit	description msds pack insert	395
928-38050	IRDye 700DX Protein Labeling Kit - Microscale Contents: 3x IRDye 700DX reactive dye vials (0.5 mg) 1x 0.5 ml 1M potassium phosphate 1x 25 ml 1X PBS 1x 0.5 ml ultra-pure water 3x desalting spin columns	1 Kit	description msds pack insert	295
928-38052	IRDye 680 Protein Labeling Kit - High Molecular Weight Contents: 3x IRDye 680 reactive dye vials (0.1 mg) 1x 0.5 ml 1M potassium phosphate 1x 25 ml 1X PBS 1x 0.5 ml ultra-pure water 3x desalting spin columns	1 Kit	description msds pack insert	350.00
928-38056	IRDye 680 Protein Labeling Kit - Microscale Contents: 3x IRDye 680 reactive dye vials (0.1 mg) 1x 0.5 ml 1M potassium phosphate 1x 25 ml 1X PBS 1x 0.5 ml ultra-pure water 3x desalting spin columns	1 Kit	description msds pack insert	295.00

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