IRDye® 800 CW

IRDye® Protein Labeling Kits label antibodies and other proteins for applications such as Western blots, In-Cell Western™ assays, in vivo imaging, and whole organ or tissue section assays. IRDye labeling kits are cost-effective alternatives to expensive, time-consuming, custom antibody labeling services.

Labeling Reactions

IRDye reactive dyes bear an N-hydroxysuccinimide (NHS) reactive group that couples to free amino groups and forms a stable conjugate. Proteins other than IgG antibodies can be labeled, but protocol adjustment and optimization may be necessary.

IRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Simply dissolve the protein in the provided buffer, combine it with the appropriate amount of water soluble amine-reactive IRDye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.

Ordering Information:

Part # DescriptionUnit/SizeDownloadsOrder Online
928-38040IRDye 800CW Protein Labeling Kit - High Molecular Weight
Contents:
  • 3x IRDye 800CW reactive dye vials (0.1 mg)
  • 1x 0.5 ml 1M potassium phosphate
  • 1x 25 ml 1X PBS
  • 1x 0.5 ml ultra-pure water
  • 3x desalting spin columns
1 kitdescription
msds
pack insert
$ 350.00
928-38042IRDye 800CW Protein Labeling Kit - Low Molecular Weight
Contents:
  • 3x IRDye 800CW reactive dye vials (0.5 mg)
  • 1x 0.5 ml 1M potassium phosphate
  • 1x 25 ml 1X PBS
  • 1x 0.5 ml ultra-pure water
  • 3x desalting spin columns
1 kitdescription
msds
pack insert
$ 395.00
928-38044IRDye 800CW Protein Labeling Kit - Microscale
Contents:
  • 3x IRDye 800CW reactive dye vials (0.1 mg)
  • 1x 0.5 ml 1M potassium phosphate
  • 1x 25 ml 1X PBS
  • 1x 0.5 ml ultra-pure water
  • 3x desalting spin columns
1 kitdescription
msds
pack insert
$ 295.00
928-38046IRDye 700DX Protein Labeling Kit - High Molecular Weight
Contents:
  • 3x IRDye 700DX reactive dye vials (0.5 mg)
  • 1x 0.5 ml 1M potassium phosphate
  • 1x 25 ml 1X PBS
  • 1x 0.5 ml ultra-pure water
  • 3x desalting spin columns
1 Kitdescription
msds
pack insert
350
928-38048IRDye 700DX Protein Labeling Kit - Low Molecular Weight
Contents:
  • 3x IRDye 700DX reactive dye vials (0.5 mg)
  • 1x 0.5 ml 1M potassium phosphate
  • 1x 25 ml 1X PBS
  • 1x 0.5 ml ultra-pure water
  • 3x desalting spin columns
1 Kitdescription
msds
pack insert
395
928-38050IRDye 700DX Protein Labeling Kit - Microscale
Contents:
  • 3x IRDye 700DX reactive dye vials (0.5 mg)
  • 1x 0.5 ml 1M potassium phosphate
  • 1x 25 ml 1X PBS
  • 1x 0.5 ml ultra-pure water
  • 3x desalting spin columns
1 Kitdescription
msds
pack insert
295
928-38052IRDye 680 Protein Labeling Kit - High Molecular Weight
Contents:
  • 3x IRDye 680 reactive dye vials (0.1 mg)
  • 1x 0.5 ml 1M potassium phosphate
  • 1x 25 ml 1X PBS
  • 1x 0.5 ml ultra-pure water
  • 3x desalting spin columns
1 Kitdescription
msds
pack insert
350.00
928-38056IRDye 680 Protein Labeling Kit - Microscale
Contents:
  • 3x IRDye 680 reactive dye vials (0.1 mg)
  • 1x 0.5 ml 1M potassium phosphate
  • 1x 25 ml 1X PBS
  • 1x 0.5 ml ultra-pure water
  • 3x desalting spin columns
1 Kitdescription
msds
pack insert
295.00

 

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