IRDye® Protein Labeling Kits label antibodies and other proteins for applications such as Western blots, In-Cell Western™ assays, in vivo imaging, and whole organ or tissue section assays. IRDye labeling kits are cost-effective alternatives to expensive, time-consuming, custom antibody labeling services.

Labeling Reactions

IRDye reactive dyes bear an N-hydroxysuccinimide (NHS) reactive group that couples to free amino groups and forms a stable conjugate. Proteins other than IgG antibodies can be labeled, but protocol adjustment and optimization may be necessary.

IRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Simply dissolve the protein in the provided buffer, combine it with the appropriate amount of water soluble amine-reactive IRDye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.

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