Smart Gel™ is a novel, ready-to-pour SDS polyacrylamide solution that polymerizes into a unique support matrix for the electrophoretic separation of denatured proteins. The proprietary chemistry of Smart Gel eliminates the need for a stacking gel and provides ultra-fine band resolution over a wide molecular weight range (3.5 kDa to 212 kDa). The gradient-like properties of the Smart Gel matrix slow the rate of progression over a wide molecular weight range. The gradient-like properties of the Smart Gel matrix show the rate of progression of proteins through the electrophoretic field, enabling the resolution of small peptides and high molecular weight proteins with the same gel. The gels are fully compatible with all standard electrophoresis equipment, SDS-PAGE staining procedures and downstream applications, including 2D electrophoresis, Western blot transfer, protein sequencing and MALDI analysis.

Each Smart Gel kit consists of a blended acrylamide solution (acrylamide, bisacrylamide, gel buffer and SDS) and a 20X Running Buffer Solution. The Smart Gel Running Buffer supplied with the kit is essential for optimal performance. Kits are available at three different acrylamide concentrations and require only the addition of APS and TEMED prior to pouring the gel.

LI-COR Smart Gel