NewBlot
- PVDF Stripping Buffer for Immobilon-FL PVDF Membranes |
Original Blot |
Antibodies used:
rabbit anti-B tubulin, IRDye 680 goat
anti-rabbit (red), mouse anti-ERK2, IRDye 800CW goat anti-mouse
(green) |
Strip #1 |
Strip #1 Preprobed with the same antibodies |
Strip #2 |
Strip #2 Preprobed with the same antibodies |
Strip #3 |
Strip #3 Preprobed with the same antibodies |
| |
|
Figure 1. Invitrogen
EPAGE-96 6% gel loaded with replicate A-431 lysate samples
and detected with anti-Syk (IRDye680, red bands) and
anti-beta-Tubulin (IRDye800CW, green bands). |
|
Figure 2. Stripped
with NewBlot-PVDF Stripping Buffer for 5 minutes at room
temperature. |
|
Figure 3. Re-probed
with anti-Syk and anti-beta-Tubulin antibodies. |
Note: all
blots have been scanned at 169 micron resolution and
scan intensity of 6 on the Odyssey Infrared Imaging System
View data:
NewBlot - Nitro
Stripping Buffer for Nitrocellulose Membranes
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