In-Cell Western: Introduction The In-Cell Western Assay, a typical workflow, basic considerations for success, and sample protocol.
In-Cell Western: Seeding Cells (Section1, Part 1) Steps and considerations for growing a successful monolayer in your microplate.
In-Cell Western: The Assay (Section 1, Part 2) How to treat, fix, permeabilize, and block the cells.
In-Cell Western: Antibody Incubation ( Section1, Part 3 and 4) Steps involved in incubating the primary and secondary antibodies.
In-Cell Western: Scanning and Grid Alignment (Section 2) Scanning, applying a grid automatically, and automatic calculations.
In-Cell Western: Grid Placement (Section 3)Automatic grid placement, moving a grid manually, resizing and rotating a grid, moving features, and changing feature size or type.
In-Cell Western: Setup Parameters (Section 4) Applying a different ICW template, temporarily changing the ICW parameters, the Well Types, Well Links, and Calculations tabs, and applying the changes.
In-Cell Western: Results (Section 5) Color codes, recalculating and exporting response data.