LI-COR Biosciences - Biotechnology Product Line

LI-COR Webinar Series

LI-COR is pleased to announce their Webinar Training and Education Series.

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Register for Part 3 Today. See details below.

FIVE-PART EDUCATIONAL WEBINAR SERIES: Optical Imaging

Many researchers are beginning to use molecular imaging tools to address their scientific questions. Molecular imaging encompasses a variety of methods, including the newer area of optical imaging.

LI-COR Biosciences has organized a multi-part series on molecular imaging to 1) provide an overview of optical imaging, target determination, agent development and optical system design; and 2) to highlight some of the considerations for integration of this technology into your research lab. (more information on optical imaging)

Part One: A Technology Introduction to Optical Imaging.

Details...

View On-Demand!

This presentation aired on April 23, 2009.

Part 2: A Technical Overview of in vivo Optical Imaging Agents & Targets

Details...

This webinar will discuss...

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Part 3: A Technical Overview of Optical Imaging: LIght Sources & Target Illumination

Speaker: Rex Peterson, Engineering Product Support Manager
When: June 25, 2009; 8:30 AM CDT or 1:30 PM CDT
Where: Online - Login info will be emailed upon registration

Part 4: A Technical Overview of Optical Imaging: Dye Absorption & Fluorescence

Details...

Part 5: A Technical Overview of Optical Imaging: Light Collection, Imaging Display & Dynamic Range

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Improving Sensitivity with Odyssey Blots vs. Chemiluminescence Blots Improve your Odyssey blot performance with the tips provided in this presentation. Troubleshooting tips include: Changing software settings such as the Background, Linear Manual/Linear Auto, and Brightness and Contrast. Altering the assay chemistry by changing dilution factors, using different blocking buffers, and more.
View On-Demand! Chemi IR™ Detection of Western Blots on the Odyssey® Infrared Imager LI-COR introduces a simple method to convert chemiluminescent Western blots to blots that can be scanned on the Odyssey Infrared Imager, making them quantitative! Speaker: Shawn Mischnick, Molecular Biologist When: May 19, 2009; 8:30 AM CDT or 1:30 PM CDT Where: Online - Login info will be emailed upon registration Panelists: Kristi Ambroz, Director of Biology Reagent Operations and Technical Support; Teresa Urlacher, Reagent Product Development Manager; Jim Wiley, Strategic Marketing Manager; Tony Endozo, Marketing Product Manager - Reagents. Description: The Chemi IR™ Detection Kit enables detection of peroxidase-conjugated antibodies with an IRDye® labeled fluorescent antibody, making western blots quantitative. A few of the benefits that can be achieved with Chemi IR detection are: Details... Bridging technology for converting chemiluminescent detection to fluorescent detection Quantitative data Significant cost savings compared to chemiluminescent detection alone Wider dynamic linear range Journal quality images and data No more darkroom Environmentally friendly method, no more fixer and developer! Equal or better sensitivity
Quantitative, Multiplexed Western Blot Detection with Infrared Fluorescence – Dr. Amy Geschwender May 1, 2008 (56 minutes) This presentation discusses the differences between fluorescent and chemiluminescent Western detection, and illustrates how this technology is being used around the world. Details... How near-infrared fluorescence differs from other Western blotting methods Linear ranges of detection for fluorescence and chemiluminescence Multiplexed Westerns Transitioning from ECL to fluorescence How this technology is being used Quantification and normalization Phosphoprotein analysis Validation of RNAi knockdown High throughput Western blotting In-gel Western detection without transfer Reagents, instruments, and software for fluorescent Western blotting

Odyssey Infrared Imaging System - Dr. Amy Geschwender - Mar 6, 2009 Previously featured on BioCompare, Dr. Geschwender, Director of Molecular Biology @ LI-COR Biosciences, introduces the Odyssey Infrared Imaging System with particular focus on it's application in Western Blotting and it's advantages when compare with the best chemiluminescence has to offer.

Characterizing GPCR Function - Dr. Christopher Kearn - Apr 16, 2009 Dr. Christopher Kearn, University of Washington, discusses the effects of CB1 and CB2 cannabinoid receptor stimulation or inhibition and how the signaling of CB1 receptors through these various effectors is regulated.

Antibody FAQs - Dr. Kristi Ambroz - Mar 6, 2009 Watch a 9 minute question and answer presentation by Dr. Kristi Ambroz, LI-COR Senior Scientist. Questions Include: • What are the advantages of infrared detection? • What kinds of dye labeled antibodies can I use on the Odyssey/Aerius? • Where do I get infrared labeled antibodies?

Optimizing Signal Detection for Odyssey Western Blots - Mar 11, 2009 A short instruction on how to troubleshoot missing and weak Odyssey Western Blot bands.

IRDye® 800CW 2-DG: A Broadly Applicable Tumor Imaging Agent for Preclinical Research
Join us for a discussion of the performance of 2-deoxy-D-glucose labeled with NIR dye, IRDye 800CW, as an optical imaging agent for in vivo imaging.

eva This presentation will discuss:

Speaker: Dr. D. Michael Olive, Vice President, Science and Technology, Biotechnology

More info on IRDye 800CW 2-DG is available here.

Why Image in the NIR?
Baylor College of Medicine Seminar Series - January 30, 2008

Instrumentation and Imaging Considerations - Dr. Eva M. Sevick-Muraca

Seminar on the value of NIR imaging and how it compares to other imaging technologies.

Systematic Evaluation and Use of Targeted IRDye labeled Optical Contrast Agents - Dr. D. Michael Olive

Seminar on the systematic development of optical agents for in vivo cancer imaging.

In Vivo Imaging of Cytotoxic T Lymphocyte Homing using LI-COR IRDye 800CW Near Infrared Dye - Dr. Aaron Foster

Seminar on the use of IRDye 800CW labeled t-cells for in vivo tracking.

Nodal Staging of HER2 Positive Breast Cancer with Dual-Labeled Trastuzumab Based Imaging Agent - Lakshmi Sampath

Seminar on the use of IRDye 800CW labeled Trastuzumab for targeting Breast cancer.

NIR Imaging With the LI-COR Pearl Imager
Univ. of Nebraska Med. Center Seminar Series - April 30th, 2008

In Vivo Tracking of the BChE/IRDye 800CW Complex in BChE Knockout Mice - Ellen Duysen

Seminar highlighting the use of the LI-COR Pearl Imager for detection of IRDye labeled BChE.

Systematic Evalutaion of Targeted IRDye Labeled Optical Imaging Agents - Joy Kovar M.S.

A thorough discussion of optical agent development using the LI-COR Pearl Imager.

Illuminating Prostate Cancer Progession- Dr. Melanie Simpson

Utilization of the LI-COR Pearl Imager to measure the progression of prostate cancer in mice.

Education Series


Advances in In Vivo Imaging: Near-Infrared Optical Imaging of Mice - Jeff Harford, LI-COR Biosciences

	Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer - Dr. Eva Sevick-Muraca and Dr. Shi Ke, Baylor College of Medicine - Mar 6, 2009

	Prostate Cancer Imaging - Dr. Melanie Simpson - Mar 6, 2009 Dr. Melanie Simpson, University of Nebraska-Lincoln, discusses the challenges of in vivo research and the use of near-infrared probes for small animal imaging.

	Near Infrared Fluorescent Approaches to Cell-Based Assays and Small Animal Imaging - Dr. Amy Schutz-Geschwender - May 1, 2007 Presented at Experimental Biology, May 1, 2007

"Inquiry-based Learning in Undergraduate Genomics Education" - Dr. Cheryl Kerfeld -

Dr. Cheryl Kerfeld discusses the value of inquiry based learning (IBL).

UCLA's "Undergraduate Genomics Research Initiative" - Dr. Cheryl Kerfeld -

Dr. Cheryl Kerfeld discusses how IBL has been implemented into the undergraduate program at UCLA.

Genomics Education Matching Funds Informative Webinar - Jackie Potts

Webinar topics include:

What the GEMF program is, and how it has been modified to
better meet your needs
How research in the classroom enhances student and faculty interaction
How this award can enhance student and faculty recruitment
How this award can help your university become recognized as a leader in
undergraduate science education
Testimonials from past GEMF winners on how they have benefited from the program.

Odyssey Power Users Guide

Part 1a – Scanning

This presentation will cover many aspects associated with the set-up, naming, and previewing of scans on the Odyssey Infrared Imager. Details...

Part 1b – Scanning

This presentation will highlight how to set and edit scan and scanner parameters. Details...

Part 1c – Scanning

This presentation will cover everything from placing a sample on the Odyssey to starting and completing a scan. Details...

Part IIa – Image Adjustment

This presentation will explain how to adjust the scan image display. Details...

Part IIb – Image Adjustment

This presentation will explain several options for image orientation and placement. Details...

Part IIc – Image Adjustment

This presentation will explain several image display options and image annotation settings. Details...

Part IIIa – Analysis

This presentation will discuss quantification and concentration calculations. Details...

Part IIIb – Analysis

In this presentation we will discuss how to draw features on a scanned image. Details...

Part IIIc – Analysis

In this presentation we will discuss how to use Plug-In reports successfully. Details...

Part IVa – Reports

In this presentation we will demonstrate how to print and export reports. Details...

Part IVb – Reports

In this presentation we will discuss how to manage report templates. Details...

Part V – Image Export

In this presentation we will discuss how to export images for analysis in other programs or submission for publication. Details...

Other

Optimizing Signal Detection for Odyssey Western Blots - Mar 11, 2009

A short instruction on how to troubleshoot missing and weak Odyssey Western Blot bands.

Odyssey Infrared Imaging System - Dr. Amy Geschwender - Mar 6, 2009 – to December 2002.

Previously featured on BioCompare, Dr. Geschwender, Director of Molecular Biology @ LI-COR Biosciences, introduces the Odyssey Infrared Imaging System with particular focus on it's application in Western Blotting and it's advantages when compare with the best chemiluminescence has to offer.

In-Cell Western (ICW) Assay
Power Users Guide

In-Cell Western: Introduction

The In-Cell Western Assay, a typical workflow, basic considerations for success, and sample protocol.

In-Cell Western: Seeding Cells
(Section1, Part 1)

Steps and considerations for growing a successful monolayer in your microplate.

In-Cell Western: The Assay
(Section 1, Part 2)

How to treat, fix, permeabilize, and block the cells.

In-Cell Western: Antibody Incubation
(Section1, Part 3 and 4)

Steps involved in incubating the primary and secondary antibodies.

In-Cell Western: Scanning and Grid Alignment
(Section 2)

Scanning, applying a grid automatically, and automatic calculations.

In-Cell Western: Grid Placement
(Section 3)

Automatic grid placement, moving a grid manually, resizing and rotating a grid, moving features, and changing feature size or type.

In-Cell Western: Setup Parameters
(Section 4)

Applying a different ICW template, temporarily changing the ICW parameters, the Well Types, Well Links, and Calculations tabs, and applying the changes.

In-Cell Western: Results
(Section 5)

Color codes, recalculating and exporting response data.

Other

The In-Cell Western Assay
Amy Schutz-Geschwender, Ph.D.
Principal Scientist, LI-COR Biosciences

This webinar was broadcase live via webex on November 20, 2008.
A replay of the webinar can be seen by following this link.

In this webinar, we will:

Discuss the technique, and how the images are captured and quantified
Review several options for cell number normalization
Compare and contrast this assay with other related assay formats, such as FACE and CASE
Discuss a variety of examples from the literature showing how In Cell Western assays are used
Describe the “On Cell Western” variation – ligand binding and receptor recycling assays
Demonstrate how cell-based assays can be used to validate and optimize optical imaging agents that are intended for in vivo imaging use
Review optimization tips for making the transition from traditional Western blotting or immunofluorescence experiments to In Cell Westerns
Conclude with a Q&A session to address your questions about this technology and how it could help you streamline your research

The In-Cell Western Assay
Amy Schutz-Geschwender, Ph.D.
Principal Scientist, LI-COR Biosciences

This webinar was broadcase live via webex on November 20, 2008.
A replay of the webinar can be seen by following this link.

In this webinar, we will:

Discuss the technique, and how the images are captured and quantified
Review several options for cell number normalization
Compare and contrast this assay with other related assay formats, such as FACE and CASE
Discuss a variety of examples from the literature showing how In Cell Western assays are used
Describe the “On Cell Western” variation – ligand binding and receptor recycling assays
Demonstrate how cell-based assays can be used to validate and optimize optical imaging agents that are intended for in vivo imaging use
Review optimization tips for making the transition from traditional Western blotting or immunofluorescence experiments to In Cell Westerns
Conclude with a Q&A session to address your questions about this technology and how it could help you streamline your research

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