Environmental
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Integrated Fluorescence Measurements with the LI-6400XT
6400-40 Leaf Chamber Fluorometer
The 6400-40 Leaf Chamber Fluorometer transforms the LI-6400XT System into the most integrated and powerful Portable Fluorescence and Gas Exchange System available.
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Field-installable, the fluorometer easily and quickly attaches to the LI-6400XT sensor head.
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Simultaneous measurement of gas exchange and fluorescence over the same leaf area.
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Complete control of the leaf environment for collection of gas exchange and fluorescence data from a single, portable unit.
- User-defined manual or automatic measurement protocols.
Stressed Plants? A new LI-COR publication illustrates why the 6400-40 Leaf Chamber Fluorometer is an ideal complement to expand the analysis capabilities of the LI-6400XT System Click here to see why.
Technologically Advanced
The Leaf Chamber Fluorometer is a pulse-amplitude modulated (PAM) fluorometer
that can be used to take measurements on both dark- and light-adapted
samples. Measured parameters include Fo, Fm, F, Fm', and Fo', and calculated
parameters include Fv, Fv/Fm, dF/Fm, qP, qN, NPQ, and ETR.
The 6400-40 provides complete control over the actinic and saturation (independently
controlled red 630 nm and blue 470 nm LEDs), measuring (red 630 nm LEDs,
modulated from 0.25 to 20 kHz), and far-red (740 nm LED for PSI excita-tion)
light.
The unique design of the 6400-40 Leaf Chamber Fluorometer eliminates the
need for fragile, awkward fiber optic light guides. Lightweight design and
low power consumption make it possible for one person to gather data quickly
and easily. Calibration information for the Leaf Chamber Fluorometer is stored
onboard, making it easy to move between different LI-6400XT
consoles.
Fluorescence Data
Actual console display showing combination A-Ci and Fluorescence curves.
Actual console display showing Fluorescence trace of the A-Ci curve above, starting at 350 ppm, to zero, and back to 1000 ppm.
Fluorescence Research Papers
An excellent article that references the use of the 6400-40 Leaf Chamber Fluorometer appeared in the December 2002 issue of Plant Physiology. The article, entitled "Temperature Response of Mesophyll Conductance. Implications for the Determination of Rubisco Enzyme Kinetics and for Limitations to Photosynthesis in Vivo" was written by Carl J. Bernacchi, Archie R. Portis, Hiromi Nakano, Susanne von Caemmerer, and Stephen P. Long.
Download
a copy here.
A more recent reference of the 6400-40 Leaf Chamber Fluorometer can be found in the Journal of the American Society for Horticultural Science: S. Kumudini, 2004. Effect of Radiation and Temperature on Cranberry Photosynthesis and Characterization of Diurnal Change in Photosynthesis. J. Amer. Soc. Hort. Sci., vol. 129, no. 1, p. 106-111
You can read the abstract of the paper or the entire paper if you have an online subscription to the journal.
