Publications: Tumor Imaging Apoptosis: Annexin V

The authors investigated real-time in vivo retinal apoptosis after laser radiation using the detection of apoptosing retinal cells 9DARC0 technique with IRDye 800CW-labeled human recombinant annexin 5. In vivo imaging was carried out with a modified confocal scanning laser ophthalmoscope (Heidelberg Retina Angiograph 2). They observed individual apoptosing spots after suprathreshold laser exposure in vivo using DARC and histologic examination. These observations are suggestive of ongoing retinal cell death, mainly in the inner nuclear layer.

Real-Time In Vivo Imaging of Retinal Cell Apoptosis after Laser Exposure
Steffen Schmitz-Valckenberg, Li Guo, Annelie Maass, William Cheung, Anthony Vugler, Stephen E. Moss, Peter M. G. Munro, Frederick W. Fitzke, and M. Francesca Cordeiro
Invest. Ophthalmol. Vis. Sci., Jun 2008; 49: 2773 - 2780.

Shah et al labeled Annexin V with an IRDye to assess apoptosis with an optical imaging analogue of Annexin V, glucose uptake with FDG-PET and cellular proliferation with FLT-PET. They suggest molecular imaging of apoptosis can accurately predict trastuzumab-induced regression of both MMTV/HER2 transgenic mouse mammary tumors and BT474 human breast cancer cell line xenografts and may warrant further exploration clinically.

Imaging Biomarkers Predict Response toAnti-HER2 (ErbB2) Therapy in Preclinical Models of Breast Cancer
Chirayu Shah,Todd W. Miller, Shelby K. Wyatt, Eliot T. McKinley, Maria Graciela Olivares, Violeta Sanchez, Donald D. Nolting, Jason R. Buck, Ping Zhao, M. Sib Ansari, Ronald M. Baldwin, John C. Gore, Rachel Schiff, Carlos L. Arteaga, and H. Charles Manning
Clin. Cancer Res. 15: 4712 (2009).

Molecular Imaging of Therapeutic Response to Epidermal Growth Factor Receptor Blockade in Colorectal Cancer
H. Charles Manning, Nipun B. Merchant, A. Coe Foutch, John M. Virostko, Shelby K. Wyatt, Chirayu Shah, Eliot T. McKinley, Jingping Xie, Nathan J. Mutic, M. Kay Washington, Bonnie LaFleur, Mohammed Noor Tantawy, Todd E. Peterson, M. Sib Ansari, Ronald M. Baldwin, Mace L. Rothenberg, Darryl J. Bornhop, John C. Gore, and Robert J. Coffey
Clin. Cancer Res., Nov 2008; 14: 7413 - 7422.

Inhibition of Mammalian Target of Rapamycin Is Required for Optimal Antitumor Effect of HER2 Inhibitors against HER2-Overexpressing Cancer Cells
Todd W. Miller, James T. Forbes, Chirayu Shah, Shelby K. Wyatt, H. Charles Manning, Maria G. Olivares, Violeta Sanchez, Teresa C. Dugger, Nara de Matos Granja, Archana Narasanna, Rebecca S. Cook, J. Phillip Kennedy, Craig W. Lindsley, and Carlos L. Arteaga
Clin. Cancer Res. 15: 7266 (2009).