Coomassie blue protein stain is a strong 700 nm fluorophore. Its fluorescence is induced upon protein binding. A common gel stain, Coomassie blue protein stain can be used for sensitive, quantitative protein detection in gels, with a linear range from ~10 ng to 20 µg.1 It has been shown to be more sensitive than white-light imaging of Coomassie staining.1, 2
Coomassie-stained SDS-PAGE gels can be imaged on any of the Odyssey® Imagers with comparable sensitivity to Sypro Ruby, but at a lower cost.1
See some published examples of protein gel imaging
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