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Coomassie Protein Gels

Data showing immunofluorescent mapping of cannibinoid CB1 and dopamine D2 receptors in the mouse brain using two-color fluorescent detection. Kearn, CS, et al

Figure 1. Unstained protein standards were loaded across a 10% SDS-PAGE gel, separated by electrophoresis and stained with Coomassie Blue. The gel was imaged with the Odyssey Classic Infrared Imaging System in the 700nm channel.

Coomassie Gels

Figure 2. Data courtesy of Dr. G. Sarath, University of Nebraska

Coomassie blue protein stain is a strong 700 nm fluorophore, and its fluorescence is induced upon protein binding.

  • Can be used for sensitive, quantitative protein detection in gels, with a linear range from ~10 ng to 20 mg1.
  • More sensitive than white-light imaging of Coomassie staining1,2.

Coomassie-stained SDS-PAGE gels can be imaged on any of the Odyssey® Imagers with comparable sensitivity to Sypro Ruby1, but at a lower cost.

Comparison of protein detection in gels stained with SyproRuby or Coomassie.

Figure 3. Mouse liver membrane and soluble proteins were separated on 2D gels, then stained with Sypro Ruby followed by Coomassie. Image analysis indicates differences in average SNR (panel G) and total spot number (panel H); * and ** denote significant differences compared to Sypro Ruby (P<0.05 and P<0.001; n=3).

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